MBD0020

DAPI ready made solution with Antifade

For Nuclear counterstain in Fluorescence Microscopy

• Synonym(s): 4′,6-Diamidino-2-phenylindole dihydrochloride, DAPI Dye, DAPI Stain, DNA Content Counterstain, 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride
• Empirical Formula (Hill Notation): C₁₆H₁₅N₅ · 2HCl
• CAS Number: 28718-90-3
• Molecular Weight: 350.25
• Beilstein: 4894417
• MDL number: MFCD00012681

Properties

• Quality Level: 200
• form: liquid
• storage condition: protect from light
• technique(s): DNA extraction: suitable; DNA sequencing: suitable; PCR: suitable
• fluorescence: λ_ex 340 nm; λ_em 488 nm (nur DAPI); λ_ex 364 nm; λ_em 454 nm (DAPI-DNA-Komplex)
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
• InChI: 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
• InChI key: FPNZBYLXNYPRLR-UHFFFAOYSA-N

Description

General description

DAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively. Most of the fluorescent dyes fade (photobleach) when exposed to excitation light. The amount of photobleaching depends on the composition of the molecule and the time of light exposure. DAPI Ready Made Solution with Antifade is a formulation that suppress photobleaching and prolong fluorescent signals.

Application

DAPI Ready Made Solution with Antifade is a glycerol based ready to use solution provided at 1μg/mL concentration in a dropper vial that can be applied directly to cells or tissue samples on microscope slides or coverslips. It is recommended to warm up at room temperature and mix by swirling the dropper vial before use. Store DAPI Ready Made Solution with antifade at -20 C protected from light.

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Biochem/physiol Actions

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Features and Benefits

• Protects dyes from fading during imaging.
• Ready-to-use liquid.
• Easy to use Dropper vial application.
• Stained sample are stable for longer time period.
• Maintains fluorescence signal-little to no quenching.

Physical form

Liquid - DAPI readymade with antifade solution is provided at 1μg/mL concentration in glycerol-PBS based solution.

Safety Information

• Hazard Statements: H412
• Precautionary Statements: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable