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MystiCq® microRNA® SYBR® Green qPCR ReadyMix

Formulation for miRNA RT-qPCR on iQ Bio-Rad platforms



Bio-Rad MyiQ
Bio-Rad iCycler iQ
Bio-Rad iQ5

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Bio-Rad MyiQ


ABI 7500 Fast, ABI ViiA 7, Strategene Mx3000P, Strategene Mx3005P, Strategene Mx4000


ABI 5700, ABI 7000, ABI 7300, ABI 7700, ABI 7900 HT , ABI 7900 HT Fast, ABI StepOne, ABI StepOnePlus


Bio-Rad CFX384, Bio-Rad CFX96, Bio-Rad MJ Chromo4, Bio-Rad MJ Opticon 2, Bio-Rad MiniOpticon, Cepheid SmartCycler, Eppendorf® Mastercycler ep realplex2 s, Illumina Eco qPCR, Qiagen Corbett Rotor-Gene 3000, Qiagen Corbett Rotor-Gene 6000, Qiagen Corbett Rotor-Gene Q, Roche LightCycler 480

storage temp.


storage temp.


storage temp.


storage temp.


General description

The MystiCq® microRNA SYBR® Green qPCR ReadyMix is one of the integral components of the MystiCq microRNA qPCR Assay System. This master mix is a specially formulated 2X concentrate with an antibody-based hot-start Taq DNA polymerase and all the optimized components needed for highly specific, reliable, and reproducible microRNA qPCR assays. Simply add the specific MystiCq microRNA qPCR Assay Primer, the MystiCq Universal PCR primer, and cDNA template generated by the MystiCq microRNA cDNA Synthesis Kit and you′re ready to go. Because there are different methods used for fluorescent normalization on different real-time PCR instruments, there is a MystiCq microRNA SYBR Green qPCR ReadyMix available for your platform.


MystiCq® microRNA SYBR® Green qPCR ReadyMix may be used in reverse transcription-quantitative polymerase chain reaction (RT-PCR) to measure miRNA expression levels in different cell lines.

Features and Benefits

  • Exclusively for use with the MystiCq microRNA qPCR Assay Primers, Control Primers, and Universal PCR primer
  • 2X concentrated for easy reaction setup with hot-start Taq, dNTPs and optimized buffer
  • Passive reference dye (if required) included at the optimal concentration
  • This master mix can readily amplify fragments between 400 and 500 bp; however, for best results, amplicon size should be limited to 80 - 200 bp


Default reaction volume is 50 μL

100RXN is packaged as 2 X 1.25 mL
500RXN is packaged as 10 X 1.25 mL
2000RXN is packaged as 1 X 50 mL

Other Notes

MystiCq® microRNA SYBR® Green qPCR ReadyMix, iQ, is compatible with the following platforms: Biorad iCycler iQ, BioRad iQ5, BioRad MyiQ.

Legal Information

MyiQ is a trademark of Bio-Rad Laboratories, Inc.
MystiCq is a registered trademark of Qiagen Beverly Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
iQ is a trademark of Bio-Rad Laboratories, Inc.

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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