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MITOISO2

Sigma-Aldrich

Mitochondria Isolation Kit

sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

NACRES:
NA.32

Quality Level

usage

sufficient for 50 applications (2-5 x 107 cells)

shipped in

dry ice

storage temp.

−20°C

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Application

Mitochondria Isolation Kit has been used in the isolation of mitochondria from Ewing′s Sarcoma Family of Tumor (ESFT) cells, blood samples from tuberculosis patients, human neuroblastoma cells and glioma cells, myotubes and HepG2 cells.
The Mitochondria Isolation Kit provides a fast and easy method for the isolation of an enriched mitochondrial fraction from cells. Most of the isolated mitochondria will contain inner and outer membranes. The kit contains extraction buffers for both functional studies and proteome profiling. Also included is a storage buffer for intact mitochondria.

Kit Components Also Available Separately

Product No.
Description
SDS

  • C2978CelLytic M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. 10 mL

  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 2.5 mL

  • C0356Protein Extraction Reagent Type 4 1 bottle

Signal Word

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Repr. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Certificate of Analysis

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Certificate of Origin

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