- MLMIR1950
All Photos(1)
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Quality Level
product line
MISSION®
form
liquid
concentration
≥1x106 VP/ml (via p24 assay)
mature sequence
CGGCCCACGGAGCUCCUAAAGA
Sanger mature/minor accession no.
Sanger microRNA accession no.
shipped in
dry ice
storage temp.
−70°C
Related Categories
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View Full Comparison1 of 4
This Item | MLMIR0067 | MLMIR0021 | MLMIR0055 |
|---|---|---|---|
| product line MISSION® | product line MISSION® | product line MISSION® | product line MISSION® |
| concentration ≥1x106 VP/ml (via p24 assay) | concentration ≥1x106 VP/ml (via p24 assay) | concentration ≥1x106 VP/ml (via p24 assay) | concentration ≥1x106 VP/ml (via p24 assay) |
| mature sequence CGGCCCACGGAGCUCCUAAAGA | mature sequence GAGGGAGGGACGGGGGCGGUGC | mature sequence CAAGCUCGUGUCUGUGGGUCCG | mature sequence CAGUGGUUUUACCCUAUGGUAG |
| Sanger mature/minor accession no. | Sanger mature/minor accession no. | Sanger mature/minor accession no. | Sanger mature/minor accession no. |
| Sanger microRNA accession no. | Sanger microRNA accession no. | Sanger microRNA accession no. | Sanger microRNA accession no. |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
General description
Sigma′s Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a proprietary algorithm. Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector. Each miRNA construct has been cloned and sequence verified. Mature microRNA sequences are obtained from miRBase.Lentiviral transduction particles are produced from sequence-verified lentiviral plasmid vectors. Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector. Co-transfection of this vector into the appropriate cell line with compatible packaging plasmids produces viral particles that can be used to transduce mammalian cells. The polymerase II promoter, elongation factor 1 alpha (EF1A), was chosen to drive miRNA expression needed for reverse transcription of viral RNA and integration of viral DNA into the host cell genome. Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory element allowing for enhanced expression of transgenes delivered by lentiviral vectors. This lentiviral vector also carries a puromycin resistance gene for selection of cells. Unlike murine-based MMLV or MSCV retroviral systems, lentiviral-based particles permit efficient infection and integration of the specific miRNA construct into differentiated and non-dividing cells, such as neurons and dendritic cells.
Other Notes
Based on miRBase V20 Mature ID
Recommended products
Two negative controls are available: NCLMIR001 and NCLMIR002
Legal Information
MISSION is a registered trademark of Sigma-Aldrich Co. LLC
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificate of Analysis
Enter Lot Number to search for Certificate of Analysis (COA).
Certificate of Origin
Enter Lot Number to search for Certificate of Origin (COO).
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