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MLS0001

Sigma-Aldrich

MISSION® LightSwitch Luciferase Assay Reagent

Fully optimized reporter system

NACRES:
NA.84

Quality Level

product line

MISSION®

storage temp.

−20°C

General description

The MISSION LightSwitch Luciferase Assay Reagent enables one-step reagent addition to measure luciferase reporter signal. The MISSION LightSwitch Luciferase Assay Reagent and the MISSION 3′UTR Lenti GoClone report constructs are a fully optimized reporter system that includes an improved reporter gene (RenSP), an optimized assay reagent, and a genome-wide collection of 3′UTR Lenti GoClone reporter constructs. The MISSION 3′UTR Lenti GoClone reporter constructs have been optimized for use with the MISSION LightSwitch Assay Reagent.

Application

MISSION® LightSwitch Luciferase Assay Reagent has been used to measure actin promoter activity.

Legal Information

MISSION is a registered trademark of Sigma-Aldrich Co. LLC
SwitchGear Genomics is a trademark of SwitchGear Genomics

Kit Components Also Available Separately

Product No.
Description
SDS

  • MISSION® LightSwitch Assay Reagent Substrate

  • MISSION® LightSwitch Assay Reagent Buffer

  • MISSION® LightSwitch Assay Reagent Solvent

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Storage Class Code

3 - Flammable liquids

Flash Point(F)

51.8 °F

Flash Point(C)

11 °C

Certificate of Analysis

Certificate of Origin

The PLA2R1-JAK2 pathway upregulates ERRa and its mitochondrial program to exert tumor-suppressive action.
Griveau A
Oncogene, 35(38), 5033-5042 (2016)
Nathan D Trinklein et al.
Genome research, 14(1), 62-66 (2004-01-07)
The alignment of full-length human cDNA sequences to the finished sequence of the human genome provides a unique opportunity to study the distribution of genes throughout the genome. By analyzing the distances between 23,752 genes, we identified a class of
Pieter Mestdagh et al.
Molecular cell, 40(5), 762-773 (2010-12-15)
The miR-17-92 microRNA cluster is often activated in cancer cells, but the identity of its targets remains elusive. Using SILAC and quantitative mass spectrometry, we examined the effects of activation of the miR-17-92 cluster on global protein expression in neuroblastoma
Janosch Hildebrand et al.
The Journal of investigative dermatology, 131(1), 20-29 (2010-09-10)
Here, we report a comprehensive investigation of changes in microRNA (miRNA) expression profiles on human keratinocyte (HK) differentiation in vitro and in vivo. We have monitored expression patterns of 377 miRNAs during calcium-induced differentiation of primary HKs, and have compared
Federico Innocenti et al.
PLoS genetics, 7(5), e1002078-e1002078 (2011-06-04)
The discovery of expression quantitative trait loci ("eQTLs") can help to unravel genetic contributions to complex traits. We identified genetic determinants of human liver gene expression variation using two independent collections of primary tissue profiled with Agilent (n = 206) and Illumina

Articles

Lenti GoClone

Validate miRNA targets and study post-transcriptional gene regulation with lentiviral 3'UTR reporters.

Genome-wide Collection of Lenti 3’UTR Reporters for Functional Validation of microRNA Targets

Sigma-Aldrich presents an article about microRNAs (miRNAs), which are a class of naturally occurring small non-coding RNA molecules that regulate a variety of developmental and physiological processes.

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