MSST0032

SILu^™Lite MAPT Microtubule-associated protein tau-441 human

Name: SILu<SUP>™</SUP>Lite MAPT Microtubule-associated protein tau-441 human
Brand: SIGMA
Price: 813 USD

recombinant, expressed in HEK 293 cells, MS Protein Standard

Synonym(s):

Neurofibrillary tangle protein, Paired helical filament-tau (PHF-tau), Pairedhelicalfilament-tau(PHF-tau), Tau-F

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MSST0032-50UG

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About This Item

UNSPSC Code:

23201100

NACRES:

NA.12

Key Documents

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biological source

human

Quality Level

200

recombinant

expressed in HEK 293 cells

tag

8-His tagged
V5 tagged

assay

≥98% (SDS-PAGE)

form

lyophilized powder

potency

≥98% Heavy amino acids incorporation efficiency by MS

mol wt

calculated mol wt 50 kDa

technique(s)

mass spectrometry (MS): suitable

suitability

suitable for mass spectrometry (internal calibrator)

UniProt accession no.

P02649

storage temp.

−20°C

Gene Information

human ... MAPT(4137)

Related Categories

Protein Mass Spectrometry

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This Item	MSST0031	SAE0076	ABN454
Sigma-Aldrich MSST0032 SILu^™Lite MAPT Microtubule-associated protein tau-441 human	Sigma-Aldrich MSST0031 SILu^™Prot MAPT Microtubule-associated protein tau-441 human	Sigma-Aldrich SAE0076 Tau-441 human	Sigma-Aldrich ABN454 Anti-Tau (T22), oligomeric Antibody
biological source human	biological source human	biological source human	biological source rabbit
assay ≥98% (SDS-PAGE)	assay ≥98% (SDS-PAGE)	assay ≥95% (SDS-PAGE)	assay -
recombinant expressed in HEK 293 cells	recombinant expressed in HEK 293 cells	recombinant expressed in HEK 293 cells	recombinant -
technique(s) mass spectrometry (MS): suitable	technique(s) mass spectrometry (MS): suitable	technique(s) cell based assay: suitable	technique(s) ELISA: suitable, dot blot: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, neutralization: suitable, western blot: suitable
mol wt calculated mol wt 50 kDa	mol wt -	mol wt calculated mol wt 50.3 kDa (including c-terminal His and V5 tags), observed mol wt 70-100 kDa by SDS-PAGE (The protein migrates as a 70-110 kDa protein on SDS-PAGE due to glycosylation)	mol wt -
suitability suitable for mass spectrometry (internal calibrator)	suitability suitable for mass spectrometry (standard)	suitability -	suitability -

General description

Tau-441 is a member of the Tau family of proteins. Tau proteins are mainly expressed in the neurons of the central nervous system where they exert a role in stabilizing microtubules, key components of axonal transport, as well as in signal transduction.Tau proteins are subject to phosphorylation and this phenomenon regulates the association of the Tau protein with the microtubules. Deposits of Alzheimer′s disease AD-associated proteins, such as hyperphosphorylated Tau, as well as other shared misfolded proteins, such as, β-amyloid precursor protein (βAPP), ubiquitin, and various chaperones and protein kinases are thought to play a pathologic role in the cognitive decline and muscular failure.3 Malfunctioning of Tau proteins is associated with microtubules disintegration and collapsing of the neuronal transport system Among other diseases, Tau forms in cerebrospinal fluid are considered a reliable biomarker for progressive supranuclear palsy, where the levels of Tau forms ratio were significantly reduced.

Immunogen

MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGLDRIRGRKLGPFEGKPIPNPLLGLDSTRTGHHHHHHHHGGQ

Biochem/physiol Actions

SILu^™Lite MAPT is a recombinant human protein expressed in human 293 cells. It consists of 481 amino acids (including C-terminal V5 and polyhistidine tags), with a calculated molecular mass of 50 kDa. SILu Lite MAPT is an analytical standard designed to be used as starting material for preparation of calibrators and controls in LC-MS applications.

Physical form

Supplied as a lyophilized powder containing phosphate buffered saline.

Legal Information

SILu is a trademark of Sigma-Aldrich Co. LLC

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000131335

046M4779V

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SILu™Lite MAPT Microtubule-associated protein tau-441 human