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  • MTOXCE3A4
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MTOXCE3A4

Sigma-Aldrich

CypExpress 3A4 Cytochrome P450 human

Synonym(s):
CP33, CP34, CYP3A, CYP3A3, CYP3A4, CYPIIIA3, CYPIIIA4, HLP, NF-25, P450 3A4, P450C3, P450PCN1
EC Number:
NACRES:
NA.77

biological source

human

recombinant

expressed in Pichia pastoris

form

dry powder

UniProt accession no.

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CYP3A4(1576)

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biological source

human

biological source

human

biological source

human

biological source

human

recombinant

expressed in Pichia pastoris

recombinant

expressed in Pichia pastoris

recombinant

expressed in Pichia pastoris

recombinant

expressed in Pichia pastoris

form

dry powder

form

dry powder

form

dry powder

form

dry powder

UniProt accession no.

P08684

UniProt accession no.

-

UniProt accession no.

P05177

UniProt accession no.

-

shipped in

dry ice

shipped in

-

shipped in

dry ice

shipped in

-

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

Biochem/physiol Actions

Cytochrome P450 enzymes are heme containing monooxygenases which are found primarily in the mammalian liver. They catalyze the oxidative metabolism of xenobiotics. This metabolism is the initial step in the biotransformation and elimination of a wide variety of drugs and environmental pollutants from the body. Ketoconazole, itraconazole, azamulin, troleandomycin and verapamil are inhibitors, whereas rifampin is an inducer of the enzyme. The isozyme CYP3A4 is a major pathway for the invivo conversion of testosterone to 6β-Hydroxytestosterone via hydroxylation.
Cytochrome P450 is a heterogeneous family of isozymes whose primary function is to oxidize small molecules, both as a function of intermediary metabolism (e.g., fatty acids) and to detoxify exogenous compounds like xenobiotics, pollutants and drug molecules. Cytochrome P450 3A4 (CYP3A4) is one of the isoforms of cytochrome P450 (P450s). CYP3A4 oxidizes more than 90% of marketed drugs through various metabolic processes.

Features and Benefits

CypExpress 3A4 is a permeabilized and stabilized dried yeast powder preparation containing full length, unmodified, human CYP 3A4 and recombinant human NADPH oxidoreductase. Using proprietary processes, CypExpress are expressed in Pichia yeast. Following optimal expression, the cells are inactivated, permeabilized and dried to a fine powder. CypExpress retains the cellular mechanisms to provide the P450 enzymes with the energy and cofactors to continue to function for long experiments, and can generate larger amounts of metabolite than mammalian microsomes or other genetically engineered expression systems. It is designed for rapid screening of drug metabolites and for scaling up metabolite generation.

Unit Definition

A reaction mixture containing 500 μM testosterone and 100 mg/mL of CypExpress 3A4 will produce 121.9 μM 6b-hydroxytestosterone in 3 hours at 30°C under the conditions described.

Physical form

Light yellow, very fine powder

Reconstitution

Per Protocol/Variable

Legal Information

6538 is a trademark of American Type Culture Collection
CypExpress is a trademark of Oxford Biomedical Research

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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