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  • MTP001
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MTP001

Sigma-Aldrich

MITOCHONDRIAL PROTEIN IP KIT

Sufficient for 50 tests

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Synonym(s):
mitochondrial protein immunoprecipitation kit
NACRES:
NA.32

form

liquid

storage temp.

−20°C

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Sigma-Aldrich

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MTP001

MITOCHONDRIAL PROTEIN IP KIT

Mitochondrial Complex I Activity Assay Kit sufficient for 100 colorimetric tests

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MAK359

Mitochondrial Complex I Activity Assay Kit

Mitochondrial Complex III Activity Assay Kit sufficient for 100 colorimetric tests

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Mitochondrial Complex III Activity Assay Kit

Mitochondrial Membrane Potential Kit sufficient for 100 fluorometric tests (flow cytometry)

Sigma-Aldrich

MAK160

Mitochondrial Membrane Potential Kit

storage temp.

−20°C

storage temp.

-

storage temp.

−20°C

storage temp.

−20°C

form

liquid

form

-

form

-

form

-

General description

The mitochondrial protein immunoprecipitation (IP) kit contains a ready-to-use mitochondria protein IP buffer, optimized for immunoprecipitation (IP and co-IP) using mitochondria and mitochondrial extracts. The buffer is a gentle formulation, which maintains the stability of mitochondrial complexes.

Application

MITOCHONDRIAL PROTEIN IP KIT has been used to perform immunoprecipitation and co-immunoprecipitation (Co-IP) of mitochondrial proteins.

Biochem/physiol Actions

The mitochondrial protein immunoprecipitation (IP) kit also provides choices of detergents, N-dodecyl-β-D-maltoside, Triton X-100, and digitonin, to achieve different stringency conditions for protein-protein interaction studies. Triton X-100 is the most commonly used detergent, especially for membrane protein solubilization. The mitochondrial protein immunoprecipitation (IP) kit may be used for the following:
  • optimized for compatibility with immunoprecipitation (IP and co-IP) and pull-down using tagged proteins
  • gentle formulation for maintenance of stable mitochondrial complexes
  • compatible with sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS PAGE), 2D gel, native gel, and mass spectrometry
  • functional assays and enzymatic assays

Signal Word

Danger

Hazard Statements

H301 - H314 - H410

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

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