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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 kit | Check Cart for Availability | $540.00 |
About This Item
NACRES:
NA.25
UNSPSC Code:
12352200
$540.00
Check Cart for Availability
General description
Gel filtration chromatography is a well-known technique for measuring the size and molecular weight of proteins. It separates proteins based on their ability to diffuse into the pores of the resin. Larger proteins have more difficulty entering the resin pores, so they flow through the column more quickly than smaller proteins. As a result, the proteins are eluted from the column in order of decreasing molecular weight.
Application
Gel Filtration Markers Kit for Protein Molecular Weights 29,000-700,000 Da has been used:
- for protein purification and analyses (gel-filtration experiments)[1]
- for size-exclusion chromatography/gel filtration chromatography[2][3] of synaptoneurosome extract[4]
- for glycerol gradient centrifugation[5]
- to study glucocorticoid-induced lymphocytolysis as a model system for apoptosis within the immune system
- to isolate Polygonatum odoratum lectin, which showed remarkable anti-HSV-II activity towards Vero cells, cytotoxicity towards human melanoma A375 cells and induced apoptosis in a caspase-dependent manner
Biochem/physiol Actions
The method described in the bulletin for determining molecular masses using gel filtration chromatography is a modified version of existing published techniques. The protein standards included in this kit may be compatible with other chromatographic systems like high-performance liquid chromatography (HPLC). However, certain buffer systems may affect the elution volumes of albumin and carbonic anhydrase. The proteins in this kit have a molecular mass range spanning from 29 kDa to 699 kDa.
Other Notes
- For research use only. Not for drug, household, or other uses.
- Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
- Please refer to the product information sheet to learn more about reagents, storage and stability, and procedure.
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) liquid chromatography (LC): suitable | technique(s) protein quantification: suitable | technique(s) liquid chromatography (LC): suitable | technique(s) - |
| form liquid | form solid | form solid | form liquid |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −70°C |
Kit Components Also Available Separately
Product No.
Description
SDS & Pricing
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| MWGF1000-1KT | 04061834076353 |




