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MWGF1000

Sigma-Aldrich

Gel Filtration Markers Kit for Protein Molecular Weights 29,000-700,000 Da

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Synonym(s):
Gel Filtration Markers Kit, Gel Filtration Protein Ladder, Protein Molecular Weight Marker Kit
NACRES:
NA.25

form

liquid

Quality Level

storage temp.

−20°C

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This Item
MWGF70MWGF200R7020
vibrant-m

R7020

Transcript RNA Markers 0.2-10 kb

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

General description

Gel filtration chromatography is a well-known technique for measuring the size and molecular weight of proteins. It separates proteins based on their ability to diffuse into the pores of the resin. Larger proteins have more difficulty entering the resin pores, so they flow through the column more quickly than smaller proteins. As a result, the proteins are eluted from the column in order of decreasing molecular weight.

Application

Gel Filtration Markers Kit for Protein Molecular Weights 29,000-700,000 Da has been used:
  • for protein purification and analyses (gel-filtration experiments)
  • for size-exclusion chromatography/gel filtration chromatography of synaptoneurosome extract
  • for glycerol gradient centrifugation
  • to study glucocorticoid-induced lymphocytolysis as a model system for apoptosis within the immune system
  • to isolate Polygonatum odoratum lectin, which showed remarkable anti-HSV-II activity towards Vero cells, cytotoxicity towards human melanoma A375 cells and induced apoptosis in a caspase-dependent manner

Biochem/physiol Actions

The method described in the bulletin for determining molecular masses using gel filtration chromatography is a modified version of existing published techniques. The protein standards included in this kit may be compatible with other chromatographic systems like high-performance liquid chromatography (HPLC). However, certain buffer systems may affect the elution volumes of albumin and carbonic anhydrase. The proteins in this kit have a molecular mass range spanning from 29 kDa to 699 kDa.

Other Notes

  • For research use only. Not for drug, household, or other uses.
  • Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
  • Please refer to the product information sheet to learn more about reagents, storage and stability, and procedure.

Kit Components Also Available Separately

Product No.
Description
SDS

  • A8531Albumin, bovine serum, 66,000 MW 1 mL/vialSDS

  • A8656Alcohol Dehydrogenase, yeast, 150,000 MW 1 mL/vialSDS

  • A8781 β-Amylase, sweet potato, 200,000 MW 1 mL/vialSDS

  • A3660 Apoferritin, horse spleen, 443,000 MW 1 mL/vialSDS

  • D4772Blue dextran, 2,000,000 MW 1 mL/vialSDS

  • C7025Carbonic Anhydrase, bovine erythrocytes, 29,000 MW 1 mL/vialSDS

  • T9145Thyroglobulin, bovine, 669,000 MW 1 mL/vialSDS

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Lei Yue et al.
Nucleic acids research, 48(17), 9589-9605 (2020-08-29)
Transcription termination defines accurate transcript 3'-ends and ensures programmed transcriptomes, making it critical to life. However, transcription termination mechanisms remain largely unknown in Archaea. Here, we reported the physiological significance of the newly identified general transcription termination factor of Archaea
Gel-filtration chromatography
F'ag'ain 'O?C, et al.
Journal of biological and chemical chronicles, 25-33 (2011)
Qi Zong Lao et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 24(12), 5013-5023 (2010-08-25)
Voltage-gated calcium channels conduct Ca(2+) ions in response to membrane depolarization. The resulting transient increase in cytoplasmic free calcium concentration is a critical trigger for the initiation of such vital responses as muscle contraction and transcription. L-type Ca(v)1.2 calcium channels
Vanessa Castro-Rodríguez et al.
BMC plant biology, 15, 20-20 (2015-01-23)
Glutamine synthetase (GS; EC: 6.3.1.2, L-glutamate: ammonia ligase ADP-forming) is a key enzyme in ammonium assimilation and metabolism in higher plants. In poplar, the GS family is organized in 4 groups of duplicated genes, 3 of which code for cytosolic
D K Wimsatt et al.
Clinical chemistry, 33(11), 2100-2106 (1987-11-01)
The Coomassie Brilliant Blue G-250 method for urinary proteins underestimates urinary immunoglobulin light chains when albumin or pooled serum is used as the protein standard. The specific color yields of these and other proteins can be brought closer together by

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