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MWGF70

Sigma-Aldrich

Gel Filtration Markers Kit for Protein Molecular Weights 6,500-66,000 Da

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Synonym(s):
Gel Filtration Protein Ladder
NACRES:
NA.25

form

solid

storage temp.

−20°C

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This Item
MWGF200R7020P9577
vibrant-m

R7020

Transcript RNA Markers 0.2-10 kb

vibrant-m

P9577

PCR 50 - 2,000 bp Marker

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

General description

Gel filtration chromatography is an established method for determining the size and molecular mass of proteins. Fractionation is based on the diffusion of molecules into the pores of the resin. Larger proteins do not enter the pores of the resin as readily but pass through the fluid volume of the column faster than smaller proteins. These protein molecules elute from the column in order of decreasing molecular mass.

Application

Gel Filtration Markers Kit for Protein Molecular Weights 6,500-66,000 Da has been used as a standard in size exclusion chromatography.
Gel filtration markers are used in protein chromatography and gel filtration chromatography. Gel filtration markers have been used to study glucocorticoid-induced lymphocytolysis as a model system for apoptosis within the immune system. Gel filtration markers have also been used to isolate Polygonatum odoratum lectin, which showed remarkable anti-HSV-II activity towards Vero cells, cytotoxicity towards human melanoma A375 cells and induced apoptosis in a caspase-dependent manner.

Kit Components Also Available Separately

Product No.
Description
SDS

  • A8531Albumin, Bovine serum, 66,000 MW 1 mL/vialSDS

  • A3886Aprotinin, Bovine lung, 6,500 MW 1 mL/vialSDS

  • D4772Blue Dextran 2,000,000 MW 1 mL/vialSDS

  • C7025Carbonic Anhydrase, Bovine erythrocytes, 29,000 MW 1 mL/vialSDS

  • C7150Cytochrome c, Horse heart, 12,400 MW 1 mL/vialSDS

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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25G
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M Zamudio et al.
Letters in applied microbiology, 32(3), 181-184 (2001-03-27)
Microbial phytases suitable for food fermentations could be obtained from lactic acid bacteria isolated from natural vegetable fermentations. Phytase activity was evaluated for six lactic acid bacteria cultures. Although the highest activity was found for Lactobacillus plantarum, the phytase activity
Gevorg Grigoryan et al.
Science (New York, N.Y.), 332(6033), 1071-1076 (2011-05-28)
There is a general need for the engineering of protein-like molecules that organize into geometrically specific superstructures on molecular surfaces, directing further functionalization to create richly textured, multilayered assemblies. Here we describe a computational approach whereby the surface properties and
Yu Chih Liu et al.
Protein expression and purification, 107, 29-34 (2014-12-03)
Helicobacter pylori infections are associated with gastritis, duodenal and gastric ulcers and gastric adenocarcinoma. Bacterial chemotaxis, mediated by four different chemoreceptors (also termed transducer-like proteins (Tlp)), plays an important role in initial colonization and development of disease. Chemoreceptor sensory domains
Comparative characterization of enzymatic digestion from fish and soybean meal from simulated digestive process of Pacific Bluefin Tuna, Thunnus orientalis
Roman-Gavilanes AI, et al.
Journal of the World Aquaculture Society, 46(4), 409-420 (2015)
Benjamin Blacklow et al.
Toxicon : official journal of the International Society on Toxinology, 55(6), 1171-1180 (2010-01-13)
Australo-papuan death adders (Acanthophis spp.) are a cause of serious envenomations in Papua New Guinea and northern Australia often resulting in neurotoxic paralysis. Furthermore, victims occasionally present with delayed-onset neurotoxicity that sometimes responds poorly to antivenom or anticholinesterase treatment. This

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