Nile Blue A is available in various forms or salts, such as sulfate, perchlorate, or chloride. Suggestion is to use the chloride form in a similar manner to the sulfate form and make adjustments based on the staining results.
N0766
Nile Blue A
Dye content, ≥75%, certified by the Biological Stain Commission, powder
Synonym(s):
Basic Blue 12, Nile blue sulfate
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About This Item
Linear Formula:
2C20H20N3O · SO4
CAS Number:
Molecular Weight:
732.85
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
222-832-5
MDL number:
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Product Name
Nile Blue A, certified by the BSC
InChI key
QIRDPEPUXNCOLD-UHFFFAOYSA-N
InChI
1S/2C20H19N3O.H2O4S/c2*1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;1-5(2,3)4/h2*5-12,21H,3-4H2,1-2H3;(H2,1,2,3,4)
SMILES string
[O-]S([O-])(=O)=O.CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)cc(N)c4ccccc34.CC\[N+](CC)=C5/C=CC6=Nc7c(OC6=C5)cc(N)c8ccccc78
agency
certified by the BSC
form
powder
composition
Dye content, ≥75%
technique(s)
microbe id | staining: suitable
color
dark green
mp
>300 °C (dec.) (lit.)
solubility
H2O: 1 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
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This Item | N5632 | N3013 | 556416 |
|---|---|---|---|
| grade certified by the Biological Stain Commission | grade - | grade technical grade | grade - |
| technique(s) microbe id | staining: suitable | technique(s) - | technique(s) titration: suitable | technique(s) microbe id | staining: suitable |
| color dark green | color - | color faint green to brown | color - |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| solubility H2O: 1 mg/mL | solubility H2O: 1 mg/mL, blue | solubility methanol: 1 mg/mL | solubility - |
| form powder | form powder | form powder | form powder, crystals or chunks |
Application
Certified for use in Lillie and Fullmer method for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue; Lillie and Fuller method for fatty acids/lipids using frozen sections of fatty liver and avocado.
Features and Benefits
Used in the preparation of an amperometric glucose sensor.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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A dual-wavelength overlapping resonance Rayleigh scattering (DWO-RRS) method was developed to detect chondroitin sulfate (CS) with nile blue sulfate (NBS). At pH 3.0-4.0 Britton-Robinson (BR) buffer medium, CS interacted with NBS to form an ion-association complex. As a result, the
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