N1780

Sigma-Aldrich

Neurotrophin-4 human

recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture, >97% (SDS-PAGE)

Synonym(s):
NTF4, NT-4
MDL number:
NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

biological source

human

recombinant

expressed in baculovirus infected Sf21 cells

assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

0.3-15.0 ng/mL EC50

mol wt

14 kDa

packaging

pkg of 5 μg

application(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... NTF4(4909)

Biochem/physiol Actions

Neurotrophin-4 (also called NT-4/5 or NT-5) is a 14 kDa member of the neurotrophin family. NT-4 shares 95% sequence homology between human and rat. NT-4 from human, rat, and Xenopus are active on chick dorsal root ganglia. NT-4 is expressed in many tissues and major brain regions. NT-4 promotes survival and differentiation of various cells in culture including spinal neuons, basal forebrain cholinergic neurons, hippocampal neurons, cerebellar granule cells, embryonic dopaminergic neruons of mesencephalon, noradrenergic neurons of the locus coeruleus, dopaminergic, GABAergic, and serotoninergic neurons of the substantia nigra, and embryonic trigeminal and jugular neurons.

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% TFA, containing 50 μg bovine serum albumin per 1 μg of cytokine.

Analysis Note

The biological activity is measured in a cell proliferation assay using a TrkB-transfected cell line BaF-TrkB-BD.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
T Timmusk et al.
The European journal of neuroscience, 5(6), 605-613 (1993-06-01)
The neurotrophin gene family includes four structurally related proteins with neurotrophic activities. Two of them, nerve growth factor and brain-derived neurotrophic factor (BDNF), have been studied in detail and information has recently emerged on the expression and function of the...
Daniel Todd et al.
PloS one, 9(2), e87923-e87923 (2014-02-08)
Huntington's disease (HD) is a devastating, genetic neurodegenerative disease caused by a tri-nucleotide expansion in exon 1 of the huntingtin gene. HD is clinically characterized by chorea, emotional and psychiatric disturbances and cognitive deficits with later symptoms including rigidity and...

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