N1905

Sigma-Aldrich

Neurotrophin-3 human

recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture, >97% (SDS-PAGE)

Synonym(s):
HDNF, NT-3, NTF3
MDL number:
NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

biological source

human

recombinant

expressed in baculovirus infected Sf21 cells

assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

1-40 ng/mL EC50

packaging

pkg of 5 μg

application(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... NTF3(4908)

General description

NTF3 (neurotrophin 3) is a neurotrophic factor belonging to the NGF (nerve growth factor) family. This protein is made up of 257 amino acids, with a prepro region of 138 amino acids and the mature sequence of 119 residues.

Application

Neurotrophin-3 human has been used in the magnetic sorting of oligodendrocyte progenitor cells.

Biochem/physiol Actions

Neurotrophin-3 (13.6 kDa or 27.2 kDa dimer) is a member of the neurotrophin family. It has identical amino acid sequence in human, mouse, and pig with full cross-reactivities. NT-3 is important in development and maintenance of neuronal populations and promotes differentiation of neural crest derived sensory and sympathetic neurons. It is critical for proprioceptive 1a afferent neurons, which relay information from peripheral muscle spindles to motoneurons, sending projections to spinocerebellar neurons. NT-3 is also critical in the superior cervical and nodose ganglia.

Physical form

Lyophilized from a 0.2 m filtered solution in phosphate buffered saline (PBS) containing 50 μg of bovine serum albumin (BSA) per 1 μg of cytokine.

Analysis Note

The biological activity is measured in a cell proliferation assay using a TrkB-transfected cell line BaF-TrkB-BD.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Neurotrophin-3 modulates breast cancer cells and the microenvironment to promote the growth of breast cancer brain metastasis
Louie E, et al.
Oncogene, 32(35), 4064-4064 (2013)
Defective glial maturation in vanishing white matter disease
Bugiani M, et al.
Journal of Neuropathology and Experimental Neurology, 70(1), 69-82 (2011)
The nerve growth factor family−neurotrophins.
Barde, Y.A. et al.
Guidebook to Cytokines and Their Receptors, 140-143 (1994)
Linkage of the gene for the triple A syndrome to chromosome 12q13 near the type II keratin gene cluster
Weber A, et al.
Human Molecular Genetics, 5(12), 2061-2066 (1996)
Neurotrophin 3 is a mitogen for cultured neural crest cells.
Kalcheim C et al
Proceedings of the National Academy of Sciences of the USA, 89(5), 1661-1665 (1992)
Articles
Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.