Neostigmine bromide

≥98% (HPLC and titration), powder

Prostigmine, 3-(N,N-Dimethylcarbamoyloxy)-N,N,N,-trimethylanilinium bromide
Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level


≥98% (HPLC and titration)






175-177 °C (lit.)


H2O: 1 g/mL
ethanol: soluble

SMILES string




InChI key


Gene Information

human ... ACHE(43)

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Neostigmine bromide has been used as an acetyl cholinesterase (AChE) inhibitor:
  • to control reaction specificity of acetylcholinesterase in coronal organ of Botryllus schlosseri.
  • to study its effects on abnormal contraction of pyloric circular smooth muscle strips (PCSMS).
  • to study its effects on depolarization of bag cell neurons.


1, 5, 10 g in poly bottle

Biochem/physiol Actions

Neostigmine bromide is a quaternary amine. It is involved in elongating and boosting the muscarinic and nicotinic effects of acetylcholine by inhibiting cholinesterase activity. It is used in anesthesia to reverse the neuromuscular blockade produced by competitive neuromuscular blockers.
Reversible inhibitor of acetylcholinesterase that is similar to eserine, but does not cross the blood-brain barrier.

Features and Benefits

This compound is also offered as part of Sigma′s Library of Pharmacologically Active Compounds (LOPAC®1280), a biologically annotated collection of high-quality, ready-to-screen compounds. Click here to learn more.

Legal Information

LOPAC is a registered trademark of Sigma-Aldrich Co. LLC


Skull and crossbonesHealth hazard

Signal Word


Target Organs

Respiratory system

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


UN 2811 6.1 / PGII

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Lucia Manni et al.
Integrative and comparative biology, 58(2), 317-328 (2018-06-07)
Tunicates, the sister group of vertebrates, possess a mechanoreceptor organ, the coronal organ, which is considered the best candidate to address the controversial issue of vertebrate hair cell evolution. The organ, located at the base of the oral siphon, controls...
Zhenyu Wu et al.
Evidence-based complementary and alternative medicine : eCAM, 2016, 5616905-5616905 (2016-07-19)
This study was designed to investigate the effect of Aurantii fructus immaturus flavonoid (AFIF) on the contraction of isolated gastric smooth muscle in rats and explore its underlying mechanisms. Isolated antral longitudinal smooth muscle strip (ALSMS) and pyloric circular smooth...
Modified carbon paste sensor for the potentiometric determination of neostigmine bromide in pharmaceutical formulations, human plasma and urine
Khorshid, A F and Issa, Y M
Biosensors And Bioelectronics, 51, 143-149 (2014)
Differentiation and induced sensorial alteration of the coronal organ in the asexual life of a tunicate
Manni L, et al.
Integrative and Comparative Biology, 58(2), 317-328 (2018)
Effect of aurantii fructus immaturus flavonoid on the contraction of isolated gastric smooth muscle strips in rats
Wu Z, et al.
Evidence-Based Complementary and Alternative Medicine : ECAM, 2016(2), 317-328 (2016)

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