N2250

Naphthol AS-BI phosphate disodium salt hydrate

Name: Naphthol AS-BI phosphate disodium salt hydrate
Brand: SIGMA
Price: 80.1 USD

fluorogenic, ≥96.0% (HPLC), powder

Synonym(s):

2-Naphthalenecarboxamide, 7-bromo-N-(2-methoxyphenyl)-3-(phosphonooxy)-, disodium salt hydrate

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$234.00

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$397.00

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About This Item

Empirical Formula (Hill Notation):

C₁₈H₁₃BrNNa₂O₆P · xH₂O

CAS Number:

207569-04-8

Molecular Weight:

496.16 (anhydrous basis)

UNSPSC Code:

12352204

PubChem Substance ID:

24897558

NACRES:

NA.83

Key Documents

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Product Name

Naphthol AS-BI phosphate disodium salt hydrate, ≥96.0% (HPLC)

SMILES string

O.[Na+].[Na+].COc1ccccc1NC(=O)c2cc3cc(Br)ccc3cc2OP([O-])([O-])=O

InChI

1S/C18H15BrNO6P.2Na.H2O/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24;;;/h2-10H,1H3,(H,20,21)(H2,22,23,24);;;1H2/q;2*+1;/p-2

InChI key

WNPKMQCBZKBNOS-UHFFFAOYSA-L

assay

≥96.0% (HPLC)

form

powder

solubility

water: 50 mg/mL, clear, colorless to yellow

storage temp.

−20°C

Quality Level

200

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Phosphates

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Show Differences

1 of 4

This Item	N6125	N2125	N4006
Sigma-Aldrich N2250 Naphthol AS-BI phosphate disodium salt hydrate	Sigma-Aldrich N6125 Naphthol AS-TR phosphate disodium salt	Sigma-Aldrich N2125 Naphthol AS-BI phosphate	Sigma-Aldrich N4006 Naphthol AS-BI N-acetyl-β-D-glucosaminide
assay ≥96.0% (HPLC)	assay ≥99% (HPLC)	assay ≥98% (HPLC)	assay ≥97% (TLC)
solubility water: 50 mg/mL, clear, colorless to yellow	solubility water: 50 mg/mL, clear, colorless to faintly yellow	solubility methanol: 50 mg/mL	solubility DMF: 50 mg/mL
Quality Level 200	Quality Level 300	Quality Level 200	Quality Level 200
form powder	form powder	form powder	form powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

General description

Naphthol AS-BI (7-Bromo-3-hydroxy-2-naphtho-o-anisidine) is a substituted naphthol.[1]

Substrate for the histochemical demonstration of acid and alkaline phosphatase.

Application

Naphthol AS-BI phosphate disodium salt hydrate is suitable to use:

in tartrate-resistant acid phosphatase (TRAP) staining to detect osteoclast activity[2]
as a component in Tris-HCl in staining cells to measure alkaline phosphatase (ALP) activity[3]
to perform alkaline phosphatase reaction[4]

Biochem/physiol Actions

Naphthol AS-BI (7-Bromo-3-hydroxy-2-naphtho-o-anisidine) can be used as a substrate to determine alkaline and acid phosphatase. It is also used to determine the activity of acid phosphatase in single cells.[1]

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Naphthol AS-BI (7-bromo-3-hydroxy-2-naphtho-o-anisidine) phosphatase and naphthol AS-BI beta-D-glucuronidase in Chinese hamster ovary cells: biochemical and flow cytometric studies.

Dolbeare FA and Phares W

The Journal of Histochemistry and Cytochemistry, 27(1), 120-124 (1979)

Establishment of human cementifying fibroma cell lines by transfection with temperature-sensitive simian virus-40 T-antigen gene and hTERT gene

Kudo Y, et al.

Bone, 30(5), 712-717 (2002)

Effects of estrogen deficiency on microstructural changes in rat alveolar bone proper and periodontal ligament

Liu Z, et al.

Molecular Medicine Reports, 12(3), 3508-3514 (2015)

Enhanced fibrillin-2 expression is a general feature of wound healing and sclerosis: potential alteration of cell attachment and storage of TGF-beta

Brinckmann J, et al.

Laboratory Investigation; a Journal of Technical Methods and Pathology, 90(5), 739-752 (2010)

A Mouse Model for Studying the Development of Apical Periodontitis with Age.

Elisheva Goldman et al.

Cells, 10(3) (2021-04-04)

Older age is associated with reduced immune function. Our aim was to study how age affects the development of apical periodontitis (AP). AP was induced in two age groups of mice (young vs. adult). Histological samples were stained by Hematoxylin

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Naphthol AS-BI phosphate disodium salt hydrate