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N2513

Sigma-Aldrich

NGF-β from rat

recombinant, expressed in Sf21 cells, lyophilized powder, suitable for cell culture

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Synonym(s):
Nerve Growth Factor-β from rat
CAS Number:
MDL number:
NACRES:
NA.77

biological source

rat

Quality Level

recombinant

expressed in Sf21 cells

assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

0.15-3.00 ng/mL EC50

packaging

pkg of 0.1 mg

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

solubility

water: soluble 0.1 g/L at 20 °C

UniProt accession no.

storage temp.

−20°C

Gene Information

rat ... NGF(4803)

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This Item
SRP3018SRP3015SRP3238
vibrant-m

N2513

NGF-β from rat

vibrant-m

SRP3018

Beta -NGF human

vibrant-m

SRP3015

Beta NGF human

vibrant-m

SRP3238

EGF from rat

biological source

rat

biological source

human

biological source

human

biological source

rat

assay

≥97% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

-

impurities

endotoxin, tested

impurities

<0.1 EU/μg endotoxin, tested

impurities

<0.1 EU/μg endotoxin, tested

impurities

<0.1 EU/μg endotoxin, tested

General description

NGFβ (nerve growth factor β) is a protein involved in signal transduction, and expressed by multiple tissues such as placenta, urothelium and bladder smooth muscle cells. It is a neurotrophin and belongs to the NGF family.

Application

NGF-β from rat is used for the following applications:
  • In the preparation of DMEM medium (for the cultivation and differentiation of PC12 cells)
  • Western blotting analysis
  • For the preparation of differentiation medium (it is prepared from DMEM, in which NGF-β is one of the components)
  • In cell culture and treatment (for neuronal differentiation)
  • In the preparation of stock solution

Biochem/physiol Actions

NGFβ (nerve growth factor β) is essential for the differentiation and survival of neurons. It also plays a key role in the development and maintenance of nervous system. It is also involved in angiogenesis, and facilitates tumorigenesis such as, oral, breast and prostate cancers. It has potential as marker for the diagnosis of interstitial cystitis/painful bladder syndrome (IC/PBS) and overactive bladder (OAB), and might also aid in determining the therapy for the same. It might be involved in changes in placental function, as NGF signaling is influenced by antenatal depression and selective serotonin reuptake inhibitor (SSRI) treatment.

Physical form

Lyophilized from a 0.2 μm filtered solution in PBS and 1 M NaCl containing 50 μg of bovine serum albumin per 1 μg of cytokine

Analysis Note

The biological activity is measured in a cell proliferation assay using the factor-dependent human erythroleukemic cell line, TF-1.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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To examine whether urinary nerve growth factor (NGF) could serve as a biomarker for interstitial cystitis/painful bladder syndrome (IC/PBS), we conducted a comprehensive meta-analysis of 9 studies. Among the studies considered, patients with IC/PBS had higher urinary NGF and NGF/Cr

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