N2513

Nerve Growth Factor β rat

≥97% (SDS-PAGE), recombinant, expressed in Sf21 cells, lyophilized powder, suitable for cell culture

• Synonym(s): NGF-β from rat, Nerve Growth Factor-β from rat
• CAS Number: 86923-98-0
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00164982
• Biological source: rat
• Recombinant: expressed in Sf21 cells
• Assay: ≥97% (SDS-PAGE)
• Form: lyophilized powder
• Impurities: endotoxin, tested

Properties

• Product Name: NGF-β from rat, recombinant, expressed in Sf21 cells, lyophilized powder, suitable for cell culture
• biological source: rat
• Quality Segment: 200
• recombinant: expressed in Sf21 cells
• assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.15-3.00 ng/mL EC₅₀
• packaging: pkg of 0.1 mg
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• solubility: water: soluble 0.1 g/L at 20 °C
• UniProt accession no.: P25427
• storage temp.: −20°C
• Gene Information: rat ... NGF(4803)

Description

General description

NGFβ (nerve growth factor β) is a protein involved in signal transduction, and expressed by multiple tissues such as placenta, urothelium and bladder smooth muscle cells.[1][2] It is a neurotrophin and belongs to the NGF family.[3]

Application

NGF-β from rat is used for the following applications:

• In the preparation of DMEM medium (for the cultivation and differentiation of PC12 cells)[4][5]
• Western blotting analysis[6]
• For the preparation of differentiation medium (it is prepared from DMEM, in which NGF-β is one of the components)[7]
• In cell culture and treatment (for neuronal differentiation)[8]
• In the preparation of stock solution[9]

Biochem/physiol Actions

NGFβ (nerve growth factor β) is essential for the differentiation and survival of neurons. It also plays a key role in the development and maintenance of nervous system. It is also involved in angiogenesis, and facilitates tumorigenesis such as, oral, breast and prostate cancers.[3] It has potential as marker for the diagnosis of interstitial cystitis/painful bladder syndrome (IC/PBS) and overactive bladder (OAB), and might also aid in determining the therapy for the same.[1] It might be involved in changes in placental function, as NGF signaling is influenced by antenatal depression and selective serotonin reuptake inhibitor (SSRI) treatment.[2]

Physical form

Lyophilized from a 0.2 μm filtered solution in PBS and 1 M NaCl containing 50 μg of bovine serum albumin per 1 μg of cytokine

Analysis Note

The biological activity is measured in a cell proliferation assay using the factor-dependent human erythroleukemic cell line, TF-1.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)