Each set priced at N1895 will prepare 5ml of solution, while each set priced at N2770 will prepare 20ml of solution.
N2770
p-Nitrophenyl phosphate
SIGMAFAST™, chromogenic, tablet
Synonym(s):
pNPP tablets
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| Size/SKU | Availability | Price |
|---|---|---|
5 sets | Available to ship TODAYfromMILWAUKEE | $74.70 |
50 sets | Estimated to ship onJune 19, 2026fromMILWAUKEE | $338.00 |
About This Item
$74.70
Available to ship TODAYDetails
Product Name
SIGMAFAST™ p-Nitrophenyl phosphate Tablets, tablet, To prepare 20 mL
Quality Segment
form
tablet
solubility
water: 0.25 tablet/mL, clear, colorless
storage temp.
−20°C
Application
Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each SIGMAFAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMAFAST™ require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.
Preparation Note
Each tablet set dissolved in 20 mL deionized water yields a ready-to-use buffered solution containing pNPP.
Legal Information
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
1 of 1
This Item | |||
|---|---|---|---|
| solubility water: 0.25 tablet/mL, clear, colorless | solubility deionized water: soluble 5 mL | solubility - | solubility water: 1 tablet/mL, clear to hazy, colorless to faintly yellow |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form tablet | form tablet | form tablet | form tablet |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Unless otherwise stated, this product is intended for research use only and is not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses, or any type of consumption or application to humans or animals.
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