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MilliporeSigma

N3393

Naphthol Blue Black

BioReagent, suitable for electrophoresis

Synonym(s):

Acid Black 1, Amido Black 10B, Buffalo Black NBR

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N3393-25G

$60.50

N3393-100G

$164.00

$60.50

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About This Item

Empirical Formula (Hill Notation):
C22H14N6Na2O9S2
CAS Number:
1064-48-8
Molecular Weight:
616.49
Colour Index Number:
20470
Beilstein/REAXYS Number:
5374263
EC Number:
213-903-1
MDL number:
MFCD00004017
UNSPSC Code:
41105322
PubChem Substance ID:
24897642
NACRES:
NA.32

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product line

BioReagent

Quality Level

200

form

powder

composition

Dye content, ~85%

technique(s)

protein staining: suitable

impurities

≤10% water

suitability

suitable for electrophoresis

SMILES string

[Na+].[Na+].Nc1c(\N=N\c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\N=N\c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;

InChI key

AOMZHDJXSYHPKS-DROYEMJCSA-L

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1 of 4

This Item
N9002195243211842
Naphthol Blue Black BioReagent, suitable for electrophoresis

Sigma-Aldrich

N3393

Naphthol Blue Black

Naphthol Blue Black Dye content ~50 %

Sigma-Aldrich

N9002

Naphthol Blue Black

Naphthol Blue Black Dye content 80 %

Sigma-Aldrich

195243

Naphthol Blue Black

Brilliant Black BN Dye content 60 %

Sigma-Aldrich

211842

Brilliant Black BN

suitability

suitable for electrophoresis

suitability

-

suitability

-

suitability

-

technique(s)

protein staining: suitable

technique(s)

-

technique(s)

-

technique(s)

-

form

powder

form

powder

form

powder

form

powder

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

composition

Dye content, ~85%

composition

Dye content, ~50%

composition

Dye content, 80%

composition

Dye content, 60%

product line

BioReagent

product line

-

product line

-

product line

-

Application

Naphthol Blue Black has been used for the staining of western blot membrane for detection of all protein that are transferred to the membrane.[1][2]

Biochem/physiol Actions

Naphthol blue black dye can be used to stain proteins on polyacrylamide gels, agarose gels and nitrocellulose membranes. After electrophoresis, fixing the proteins in the gel is recommended. The gel can then be stained with 0.1% Naphthol Blue Black in 7% (v/v) acetic acid for at least 2 hr and destained with a soluion of 7% (v/v) acetic acid. Detection sensitivity is approx. 20% that of Coomassie Blue R.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1B

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT1497
SHBS6396
SHBR8149
MKCG6768
MKCC3457

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PopF1 and PopF2, two proteins secreted by the type III protein secretion system of Ralstonia solanacearum, are translocators belonging to the HrpF/NopX family.
Meyer D, et al.
Journal of Bacteriology, 188, 4903-4917 (2006)
Disc electrophoresis of proteins in the presence of sodium dodecyl sulphate.
E De Vito et al.
Experientia, 22(2), 124-125 (1966-02-15)
M D Chengalroyen et al.
World journal of microbiology & biotechnology, 29(4), 625-633 (2012-11-29)
Investigation into the biodegradative capability of several actinomycetes led to the discovery of many strains possessing an ability to decolorize a variety of azo and triphenylmethane dyes. Of particular interest is an Amycolatopsis sp. isolate which displayed the ability to
Shih-Hsien Chang et al.
Journal of hazardous materials, 166(2-3), 1279-1288 (2009-01-23)
Degradation of Remazol Brilliant Blue R (anthraquinone dye) and Acid Black 1 (azo dye) by Fenton oxidation and low-cost Fe(0)/air process was compared. The doses of Fenton reagent needed for decolorization of Remazol Brilliant Blue R was much higher than
Teresa M Lamb et al.
G3 (Bethesda, Md.), 3(12), 2273-2280 (2013-10-22)
Precise control of gene expression is a powerful method to elucidate biological function, and protein overexpression is an important tool for industry and biochemistry. Expression of the Neurospora crassa tcu-1 gene (NCU00830), encoding a high-affinity copper transporter, is tightly controlled
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