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N3643

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NZCYM Broth

Powder microbial growth medium

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NACRES:
NA.85

grade

for molecular biology

Quality Level

sterility

non-sterile

form

powder

composition

Casamino Acids, 1 g/L
MgSO4, 0.98 g/L
NaCL, 5 g/L
Tryptone, 10 g/L
Yeast extract, 5 g/L

technique(s)

microbiological culture: suitable

pH

6.8-7.2(2.2% solution)

application(s)

food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

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This Item
L7533L3022L3522
NZCYM Broth Powder microbial growth medium

N3643

NZCYM Broth

LB Broth with agar (Lennox) EZMix® powder microbial growth medium

L7533

LB Broth with agar (Lennox)

LB Broth (Lennox) Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

L3022

LB Broth (Lennox)

Sigma-Aldrich

L3522

LB Broth (Miller)

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

form

powder

form

powder

form

powder

form

powder

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

composition

Casamino Acids, 1 g/L , NaCL, 5 g/L , Yeast extract, 5 g/L , MgSO4, 0.98 g/L , Tryptone, 10 g/L

composition

Agar, 15 g/L , NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

composition

NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

composition

NaCl, 10 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

application(s)

food and beverages
microbiology

application(s)

agriculture
food and beverages
microbiology

application(s)

food and beverages
microbiology

application(s)

food and beverages

General description

NZCYM is a microbial growth medium used for the cultivation of E. coli and lambda bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Application

NZCYM Broth has been used for non-selective cultivation of lambda, MS2, T7, CLP-P3, Phi29, and Goe1 bacteriophages, and E. coli strains for cloning, DNA plasmid production, and production of recombinant proteins. It is also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

NZCYM powder provides:
  • Standard formulation
  • Convenient package sizes
  • A budget-friendly alternative to liquid

Reconstitution

Stir to suspend 22 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Protocols

General protocols for growth of competent cells in microbial medium.

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