All Photos(4)



Anti-Neurofilament 200 antibody produced in rabbit

enhanced validation

IgG fraction of antiserum, buffered aqueous solution

NF200 Antibody - Anti-Neurofilament 200 antibody produced in rabbit, Nf200 Antibody, AntiNeurofilament heavy chain
MDL number:

biological source


Quality Level



antibody form

IgG fraction of antiserum

antibody product type

primary antibodies




buffered aqueous solution

mol wt

antigen 200 kDa

species reactivity

bovine, wide range


antibody small pack of 25 μL

enhanced validation

independent ( Antibodies)
Learn more about Antibody Enhanced Validation


immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80 using animal cerebellum sections
microarray: suitable
western blot: suitable

shipped in

dry ice

storage temp.


Gene Information

human ... NEFH(4744)
mouse ... Nefh(380684)
rat ... Nefh(24587)

General description

Neurofilament 200, also known as neurofilament heavy polypeptide (NEFH), is encoded by the gene mapped to human chromosome 22q12.2. The encoded protein has a molecular mass of 200kDa and belongs to the group of type IV intermediate filaments which are key constituents of the neuronal cytoskeleton. NEFH contains C-terminal motifs involved in the formation of sidearm projections which cross-link the neurofilaments.


Shows a wide species cross-reactivity. Reacts with neurofilaments in the central and peripheral nervous system and localizes the neurofilament polypeptide.


Neurofilament 200 from bovine spinal cord.


Anti-neurofilament 200 antibody produced in rabbit has been used for immunolabeling.
Anti-neurofilament 200 antibody produced in rabbit has been used:
  • for detection of neurofilaments using immunohistological staining
  • in human astrocytoma cell line
  • in post-mortem brain sections
  • in human cremaster muscles
  • for detection of neurofilaments by immunofluorescence staining in lumbar spinal cord sections of mice


25 μL
0.2, 0.5 mL

Biochem/physiol Actions

Neurofilament 200 plays a vital role in cytoarchitecture organization. Downregulated expression of the gene has been observed in schizophrenia patients. Loss of gene expression is associated with the development of prostate carcinoma. Mutation in the gene leads to sporadic amyotrophic lateral sclerosis (ALS).
Neurofilament 200 assembly during development is critical for normal functioning of neuronal cells. Mutations in gene encoding neurofilaments is associated with neurological disorder called the Charcot–Marie–Tooth.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at -20°C. For continuous use, the product may be stored at 2-8°C for up to one month. For extended storage, solution may be frozen in working aliquots at –20°C. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service