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N4645

Sigma-Aldrich

4-Nitrophenyl phosphate disodium salt hexahydrate

powder, BioReagent, suitable for cell culture, ≥97%

Synonym(s):
p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate
Linear Formula:
O2NC6H4OP(O)(ONa)2 · 6H2O
CAS Number:
Molecular Weight:
371.14
Beilstein:
5324661
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.75

Quality Level

biological source

synthetic (organic)

product line

BioReagent

assay

≥97%

form

powder

application(s)

cell culture | mammalian: suitable

solubility

H2O: 100 mg/mL

storage temp.

−20°C

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

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General description

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Application

4-Nitrophenyl phosphate disodium salt hexahydrate has been used:
  • as a component in diethanolamine buffer to detect alkaline phosphatase-conjugated antibodies in enzyme linked immunosorbent assay (ELISA)
  • to treat adherent cells in adhesion assay
  • as a phosphatase substrate in in vitro phosphatase assay in substrate buffer for ELISA

Packaging

1, 5 g in poly bottle

Biochem/physiol Actions

p-Nitrophenyl phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in enzyme linked immunosorbent assay (ELISA) procedures. It is the substrate of choice in alkaline phosphatase systems due to its high sensitivity. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. This product is tested for use in cell culture applications. 4-nitrophenyl phosphate (pNPP), a small-molecule substrate can be used to study on alkaline phosphatases, acid phosphatases etc.

Storage Class Code

11 - Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Serodiagnosis of Salmonella enterica serovar Typhi and S. enterica serovars Paratyphi A, B and C human infections
Chart H, et al.
Journal of Medical Microbiology, 56(9), 1161-1166 (2007)
Serodiagnosis of Echinococcus spp. infection: explorative selection of diagnostic antigens by peptide microarray
List C, et al.
PLoS Neglected Tropical Diseases, 4(8), e771-e771 (2010)
Assays for protein-tyrosine phosphatases.
McCain DF and Zhang ZY
Methods in Enzymology, 345, 507-518 (2002)
Flexible, actin-based ridges colocalise with the beta1 integrin on the surface of melanoma cells
Poole K and Muller D
British Journal of Cancer, 92(8), 1499-1499 (2005)
Cucurbitacin I inhibits STAT3, but enhances STAT1 signaling in human cancer cells in vitro through disrupting actin filaments
Guo H, et al.
Acta Pharmacologica Sinica, 39(3), 425-425 (2018)

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