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N5254

Sigma-Aldrich

Neuraminidase Agarose from Clostridium perfringens (C. welchii)

Type VI-A, ammonium sulfate suspension

Synonym(s):

Acylneuraminyl hydrolase, Receptor-destroying enzyme, Sialidase

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1 UNIT
$419.00
10 UNITS
$925.00

About This Item

EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

$419.00


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type

Type VI-A

form

ammonium sulfate suspension

specific activity

20-60 units/g agarose

extent of labeling

0.6-1.8 units per mL gel
20-60 units per g agarose

matrix

beaded agarose

storage temp.

2-8°C

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1 of 4

This Item
N2133N5631N3001
specific activity

20-60 units/g agarose

specific activity

≥50 units/mg protein (using 4MU-NANA)

specific activity

10-20 units/mg protein (using 4MU-NANA), 3.5-8.0 units/mg protein (mucin)

specific activity

2-10 units/mg protein (mucin), 6-15 units/mg protein (using 4MU-NANA)

form

ammonium sulfate suspension

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

type

Type VI-A

type

Type X

type

Type VIII

type

Type VI

extent of labeling

0.6-1.8 units per mL gel

extent of labeling

-

extent of labeling

-

extent of labeling

-

General description

Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release.

Application

Agarose-linked neuraminidase from Clostridium perfringens can be used for the detection of total and desialylated sex hormone-binding globulin in human serum samples. [1]
Neuraminidase from Clostridium perfringens has been used in a study to assess a genetic polymorphism of human serum glycoprotein (inter-α-trypsin-inhibitor). [2] It has also been used in a study to investigate the inhibition of neuraminidase by chemically sulphated glycopeptides. [3]

Unit Definition

One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.

Physical form

Suspension in 2.0 M (NH4)2SO4 solution, pH 7.0.

Preparation Note

Prepared from Neuraminidase, Type VI (N 3001).

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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