N6009

Sigma-Aldrich

Nerve Growth Factor-2.5S from murine submaxillary gland

NGF-2.5S, lyophilized powder, suitable for cell culture

Synonym(s):
NGF-2.5S
CAS Number:
MDL number:
Pricing and availability is not currently available.

Quality Level

biological source

mouse

form

lyophilized powder

potency

0.1-30 ng/mL EC50 (using PC-12 cells)

mol wt

26.5 kDa

packaging

pkg of 4X25 μg

storage condition

avoid repeated freeze/thaw cycles

application(s)

cell culture | mammalian: suitable

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

mouse ... Ngf(18049)

Other Notes

2.5S subunit of nerve growth factor-7S (NGF-7S) is essentially the β-subunit when isolated from male mouse submaxillary glands under initially dissociative conditions by a modification of the method of Bocchini and Angeletti.

Physical form

Lyophilized from a 0.2 μm filtered solution in sodium acetate buffer.

Analysis Note

The biological activity is measured using a 3-day MTT assay.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Hidetada Matsuoka et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 68(10), 679-690 (2020-09-05)
TWIK-related acid-sensitive K+ (TASK) homomeric channels, TASK1 and TASK3, are present in PC12 cells. The channels do not heteromerize due plausibly to a lack of p11 protein. Single-channel recording reveals that most of the rat carotid body (CB) glomus cells...
Haeman Jang et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(33), 14063-14068 (2009-08-12)
One of the greatest influenza pandemic threats at this time is posed by the highly pathogenic H5N1 avian influenza viruses. To date, 61% of the 433 known human cases of H5N1 infection have proved fatal. Animals infected by H5N1 viruses...
Florian Larramendy et al.
Lab on a chip, 19(5), 875-884 (2019-02-07)
This paper addresses a nanoengineering approach to create a fully three-dimensional (3D) network of living cells, providing an advanced solution to in vitro studies on either neuronal networks or artificial organs. The concept of our work relies on stackable scaffolds...
Kathrin Gassei et al.
Reproduction (Cambridge, England), 136(4), 459-469 (2008-07-29)
The first morphological sign of testicular differentiation is the formation of testis cords. Prior to cord formation, newly specified Sertoli cells establish adhesive junctions, and condensation of somatic cells along the surface epithelium of the genital ridge occurs. Here, we...
Guohao Wang et al.
Molecular neurodegeneration, 10, 42-42 (2015-09-04)
TAR DNA-binding protein 43 (TDP-43) is a nuclear protein, but it is redistributed in the neuronal cytoplasm in both amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). Because small transgenic animal models often lack cytoplasmic TDP-43, how the cytoplasmic...

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