MilliporeSigma
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N6530

Sigma-Aldrich

N1 Medium Supplement (100×)

solution, sterile-filtered, suitable for cell culture

NACRES:
NA.77

sterility

sterile-filtered

form

solution

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

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M6895SCM081SCM013
sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

sterility

-

form

solution

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell culture | stem cell: suitable

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

impurities

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

-

storage temp.

-

Quality Level

300

Quality Level

200

Quality Level

100

Quality Level

100

General description

N1 Medium Supplement (100×) can be used as a supplement to produce complete N1 neural cell growth media or closely related media.

Application

N1 Medium Supplement (100×) has been used as:
  • a component of the proliferation medium for culturing oligodendrocyte-like cells and neuroblastoma cells
  • a supplement in the Dulbecco′s modified Eagle medium (DMEM/F12) to culture human oligodendrocytes
  • a component of the α-minimum essential medium (MEM) for culturing induced primary retinal pigment epithelium (ipRPE) monolayers

Other Notes

Contains 0.5mg/ml recombinant human insulin, 0.5mg/ml human transferrin (partially iron-saturated), 0.5μg/ml sodium selenite, 1.6mg/ml putrescine, and 0.73μg/ml progesterone.

Preparation Note

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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