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MilliporeSigma

N6530

N1 Medium Supplement (100×)

solution, sterile-filtered, suitable for cell culture

Synonym(s):

N1 Supplement

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About This Item

NACRES:
NA.77
UNSPSC Code:
12352207
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sterility

sterile-filtered

Quality Segment

form

solution

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

General description

N1 Medium Supplement (100×) can be used as a supplement to produce complete N1 neural cell growth media or closely related media.

Application

N1 Medium Supplement (100×) has been used as:
  • a component of the proliferation medium for culturing oligodendrocyte-like cells and neuroblastoma cells
  • a supplement in the Dulbecco′s modified Eagle medium (DMEM/F12) to culture human oligodendrocytes
  • a component of the α-minimum essential medium (MEM) for culturing induced primary retinal pigment epithelium (ipRPE) monolayers

Preparation Note

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

Other Notes

Contains 0.5mg/ml recombinant human insulin, 0.5mg/ml human transferrin (partially iron-saturated), 0.5μg/ml sodium selenite, 1.6mg/ml putrescine, and 0.73μg/ml progesterone.


Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



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