The concentration of this product is considered proprietary.
N7653
p-Nitrophenyl Phosphate Liquid Substrate System
alkaline phosphatase substrate, liquid
Synonym(s):
4-Nitrophenyl phosphate disodium salt solution, pNPP
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100 ML
$155.00
$155.00
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About This Item
Linear Formula:
O2NC6H4OP(O)(ONa)2
CAS Number:
Molecular Weight:
263.05
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
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Product Name
p-Nitrophenyl Phosphate Liquid Substrate System, liquid
form
liquid
shipped in
wet ice
Storage temp.
−20°C
SMILES string
[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
InChI
1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2
Inchi Key
VIYFPAMJCJLZKD-UHFFFAOYSA-L
1 of 4
This Item | P7998 | B3804 | 487655 |
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| form liquid | form liquid | form liquid | form solid |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in wet ice | shipped in - | shipped in - | shipped in ambient |
General description
The p-Nitrophenyl Phosphate (pNPP) Liquid Substrate System combines p-nitrophenyl phosphate, buffer, and the required magnesium cations in a convenient, ready-to-use, single solution reagent. Producing a soluble end product, pNPP is the substrate of choice in alkaline phosphatase enzyme immunoassays.[1]
Application
p-Nitrophenyl Phosphate Liquid Substrate System has been used as a substrate to determine the activity of alkaline phosphatase[2] in alkaline phosphatase assay.[3]
Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
Biochem/physiol Actions
Physical form
Ready-to-use.
Disclaimer
Stable at least one year when stored below 0 °C.
signalword
Danger
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral
target_organs
Kidney,Liver,Blood
Storage Class
8B - Non-combustible corrosive hazardous materials
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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There is an increasing body of literature reporting treatment failure of the currently recommended radical treatment of Plasmodium vivax infections. As P. vivax is the main malaria species outside the African continent, emerging tolerance to its radical treatment regime could
J V Jones et al.
Journal of immunological methods, 118(1), 79-84 (1989-03-10)
We have recently described a substrate amplification system, based on the method of Self, which increases the sensitivity of alkaline phosphatase (AP)-dependent enzyme-linked immunosorbent assays (ELISA) by a factor of 30-50. This increase is achieved by having AP, the primary
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What is the concentration of Product N7653, p-Nitrophenyl Phosphate Liquid Substrate System?
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What type of application can Product N7653, p-Nitrophenyl Phosphate Liquid Substrate System, be used in?
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This product is recommended for ELISA (microwell) procedures and is not recommended for membrane applications.
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How should Product N7653, p-Nitrophenyl Phosphate Liquid Substrate System, be used?
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Information on usage is found in our product information sheet (under Documents, above).
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