N8130

Sigma-Aldrich

4-Nitrophenyl acetate

esterase substrate

Synonym(s):
Acetic acid 4-nitrophenyl ester
Linear Formula:
CH3CO2C6H4NO2
CAS Number:
Molecular Weight:
181.15
Beilstein/REAXYS Number:
515874
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.32

Quality Level

assay

≥98% (TLC)

form

powder or crystals

mp

75-77 °C (lit.)

storage temp.

−20°C

SMILES string

CC(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C8H7NO4/c1-6(10)13-8-4-2-7(3-5-8)9(11)12/h2-5H,1H3

InChI key

QAUUDNIGJSLPSX-UHFFFAOYSA-N

Gene Information

human ... PON1(5444)

Looking for similar products? Visit Product Comparison Guide

Application

4-Nitrophenyl acetate has been used:
  • to evaluate esterase activity in human embryonic kidney cell line
  • in carbonic anhydrase inhibition assay
  • to study its conversion to p-nitrophenol (p-NP) by the esterase activity of bovine serum albumin
  • as a substrate to determine carboxylesterase (CES) activity in mouse liver cells
  • to study the advantages of bioconjugation of cross-linked enzyme aggregates (CLEAs) and magnetic iron oxide nanoparticles (MIONPs) in magnetic CLEAs matrix

Packaging

5, 10, 25, 100 g in poly bottle

Substrates

Chromogenic esterase substrate.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Emmanuel Vázquez-Mayorga et al.
International journal of molecular sciences, 17(8) (2016-08-25)
Mutations the in human DJ-1 (hDJ-1) gene are associated with early-onset autosomal recessive forms of Parkinson's disease (PD). hDJ-1/parkinsonism associated deglycase (PARK7) is a cytoprotective multi-functional protein that contains a conserved cysteine-protease domain. Given that cysteine-proteases can act on both...
Immunomodulatory, antiglycation and anti-ulcerative properties of Ruellia squarrosa Fenzl Acanthaceae
Afzal K, et al.
Tropical Journal of Pharmaceutical Research, 16(8), 1751-1755 (2017)
Michelle R Baker et al.
Biochemistry research international, 2011, 739712-739712 (2011-09-16)
Persistent alteration of protein conformation due to interaction with isoflurane may be a novel molecular aspect of preconditioning. We preincubated human serum albumin with isoflurane, dialyzed to release agent, and assessed protein conformation. Susceptibility to chemical modification by methylglyoxal and...
Impact of diet on irinotecan toxicity in mice
Mallick P, et al.
Chemico-Biological Interactions, 291, 87-94 (2018)
Beyond esterase-like activity of serum albumin. Histidine-(nitro) phenol radical formation in conversion cascade of p-nitrophenyl acetate and the role of infrared light
Kowacz M and Warszynski P
arXiv (2018)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.