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MilliporeSigma

N9389

Sigma-Aldrich

4-Nitrophenyl phosphate disodium salt hexahydrate

chromogenic, tablet

Synonym(s):

p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate

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50 TABS
$230.00
100 TABS
$393.00

$230.00


In StockDetails

Diagnostics Manufacturers should use alternative grade product: SRE0026

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50 TABS
$230.00
100 TABS
$393.00

About This Item

Linear Formula:
O2NC6H4OP(O)(ONa)2 · 6H2O
CAS Number:
Molecular Weight:
371.14
Beilstein/REAXYS Number:
5324661
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83

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$230.00


In StockDetails

Diagnostics Manufacturers should use alternative grade product: SRE0026

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

4-Nitrophenyl phosphate disodium salt hexahydrate, tablet

description

5 mg substrate per tablet

Quality Level

form

tablet

fluorescence

λex 310 nm (pH 7.1)
λex 399 nm (Reaction Product)

storage temp.

−20°C

SMILES string

O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2

InChI key

KAKKHKRHCKCAGH-UHFFFAOYSA-L

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1 of 4

This Item
N2765N464571768
Quality Level

300

Quality Level

300

Quality Level

200

Quality Level

100

form

tablet

form

tablet

form

powder

form

powder or crystals

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

fluorescence

λex 310 nm (pH 7.1)

fluorescence

-

fluorescence

-

fluorescence

-

description

5 mg substrate per tablet

description

-

description

-

description

-

General description

Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.

Application

4-Nitrophenyl phosphate disodium salt hexahydrate has been used in:
  • enzyme-linked immunosorbent assay (ELISA) to visualize glycoprotein VI (GPVI) binding to collagen type I (Col I)[1]
  • enzymatic color reaction for semi-quantitative measurement of allergen-specific IgE by ELISA[2]
  • alkaline phosphatase assay[3]

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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