N9528

Anti-Neuropeptide Y (NPY) antibody produced in rabbit

whole antiserum

• Synonym(s): NPY Antibody - Anti-Neuropeptide Y (NPY) antibody produced in rabbit, Npy Antibody
• MDL number: MFCD00162717
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: whole antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• contains: 15 mM sodium azide
• species reactivity: rat, sheep, pig, human
• packaging: antibody small pack of 25 μL
• technique(s): immunohistochemistry (frozen sections): 1:8,000-1:16,000 using 4% paraformaldehyde perfusion-fixed sections of rat brain; indirect ELISA: 1:5,000-1:10,000 using using peptide coating at 0.5-1 μg/well in 0.05 M carbonate/bicarbonate buffer, pH 9.6, and pNPP as substrate
• UniProt accession no.: P01303
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: rat ... Npy(24604)

Description

General description

Anti-Neuropeptide Y (NPY) is developed in rabbits using synthetic neuropeptide Y (porcine) conjugated to keyhole limpet hemocyanin (KLH) as the immunogen.

Neuropeptide Y (NPY), a 36-amino acid peptide amide, is a major regulatory neuropeptide widely distributed in the mammalian central (CNS) and peripheral nervous systems (PNS). NPY belongs to the pancreatic polypeptide family of peptides which are characterized by a common tertiary structure. Within this family, an intestinal peptide hormone, peptide YY (PYY), is most closely related to NPY. In the CNS, NPY is involved in regulation of blood pressure, memory processing, circadian rhythm, and stimulation of food intake. In the PNS, NPY has potent vasoconstrictor activity and acts as a neurotransmitter/neuromodulator of sympathetic neurons and adrenal glands. NPY is one of the most abundant peptides found in the CNS, widely distributed in the brain. High levels of NPY are present in the cerebral cortex, amygdaloid nuclei, hippocampal formation, and hypothalamus. In the PNS, NPY is found mainly in sympathetic neurons that innervate vascular smooth muscle, heart, and urogenital tract. The biological actions of NPY in the brain and periphery are mediated by at least two different NPY receptors, designated Y1 and Y2 receptor subtypes.

Specificity

The antibody recognizes by ELISA, NPY (human), NPY (porcine), NPY (sheep), NPY (13-36), NPY (18-36), and cross-reacts with PYY (human). It does not cross-react with neurokinin B (NKB), neurokinin A (NKA), substance P (SP), calcitonin, somatostatin and BSA.

Immunogen

synthetic neuropeptide Y (NPY, porcine), conjugated to KLH. The corresponding sequence is highly conserved (single amino acid substitution) in human and rat NPY and is highly conserved (84% identity) in the C-terminus region (24-36) of human peptide YY.

Application

Anti-Neuropeptide Y (NPY) antibody produced in rabbit has been used in:

• single-labeling immunocytochemistry
• immunofluorescence
• immunostaining
• dot blot
• radioimmunoassay (RIA)
• enzyme-linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Neuropeptide Y (NPY) plays a vital role in feeding behaviour, anxiety, epilepsy, circadian, rhythm memory processing, sleep, pain and drug addiction. In addition, it is also implicated in regulation of appetite, body weight and obesity. It controls central endocrine secretion and has potent vasoactive effects on the cardiovascular system. NPY is involved in the pathogenesis of atherosclerosis and coronary artery disease.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable