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NA0400

Sigma-Aldrich

GenElute HP Endotoxin-Free Plasmid Maxiprep Kit

greener alternative

sufficient for 10 preparations

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Synonym(s):
Gen Elute, GenElute Endotoxin-Free Plasmid Kit
NACRES:
NA.52

usage

sufficient for 10 preparations

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

technique(s)

DNA extraction: suitable

greener alternative category

storage temp.

15-25°C

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This Item
NA0400SNA0410NA0600
usage

sufficient for 10 preparations

usage

sufficient for 4 preparations

usage

sufficient for 25 preparations

usage

 kit sufficient for 5 preparations

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

NA0400S

NA0400, NA0410

NA0400, NA0400S

-

greener alternative category

Aligned,

greener alternative category

Aligned,

greener alternative category

Aligned,

greener alternative category

, Aligned

storage temp.

15-25°C

storage temp.

-

storage temp.

15-25°C

storage temp.

15-25°C

General description

The GenElute HP Endotoxin-Free Plasmid Maxiprep Kit offers a simple and rapid method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. The kit uses a vacuum format with a filter column for the rapid clearing of the bacterial lysate and a silica column for capturing plasmid DNA. A proprietary solution binds plasmid DNA to the binding column while preventing endotoxins from adsorbing. The technology allows for the user to consistently achieve levels of endotoxin that are less than 0.1 endotoxin units per μg of plasmid DNA.

High-quality, endotoxin-free DNA is ready for immediate use for the most demanding applications including transfection with endotoxin-sensitive cells.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Features and Benefits

  • Up to 1.2 mg of high-copy plasmid DNA with endotoxin levels of ≤0.1 EU/μg
  • Only 35 minutes from pelleted cells to purified plasmid
  • Convenient vacuum format

Principle

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis. The lysate is clarified by filtration followed by the addition of a binding solution that has been optimized for endotoxin-free plasmid preparations. The plasmid DNA is then captured on silica, while endotoxins are prevented from adsorbing to the membrane. Two wash steps remove contaminants. Finally, the bound DNA is eluted in endotoxin-free water. The recovered plasmid DNA is predominately in its supercoiled form. Genomic DNA and RNA are below detectable levels by ethidium bromide stained agarose gel electrophoresis.

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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João M Furtado et al.
Investigative ophthalmology & visual science, 53(11), 6856-6862 (2012-09-07)
Toxoplasma gondii, the parasite responsible for ocular toxoplasmosis, accesses the retina from the bloodstream. We investigated the dendritic cell as a potential taxi for T. gondii tachyzoites moving across the human retinal endothelium, and examined the participation of adhesion molecules
Keith Hansen et al.
Journal of visualized experiments : JoVE, (64)(64), e3304-e3304 (2012-06-27)
Genome editing is a powerful technique that can be used to elucidate gene function and the genetic basis of disease. Traditional gene editing methods such as chemical-based mutagenesis or random integration of DNA sequences confer indiscriminate genetic changes in an
Dale Cowley et al.
eLife, 4 (2015-09-04)
The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010
Ming-Wei Chen et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(36), 13538-13543 (2008-09-04)
H5N1 influenza viruses have spread extensively among wild birds and domestic poultry. Cross-species transmission of these viruses to humans has been documented in over 380 cases, with a mortality rate of approximately 60%. There is great concern that a H5N1
Tri-Hung Nguyen et al.
International journal of oncology, 41(3), 829-838 (2012-06-14)
Overexpression of TMPRSS4, a cell surface-associated transmembrane serine protease, has been reported in pancreatic, colorectal and thyroid cancers, and has been implicated in tumor cell migration and metastasis. Few reports have investigated both TMPRSS4 gene expression levels and the protein

Related Content

Endotoxin-free Plasmid DNA Purification

Perform rapid isolation of endotoxin-free plasmid DNA using a streamlined procedure that can be completed in as fast as 35 minutes. The GenElute HP Endotoxin-Free Plasmid Maxiprep Kit delivers high plasmid recoveries with levels of endotoxin that are consistently below the industry standard of 0.1 EU/µg.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service