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GenElute HP Select Plasmid Gigaprep Kit

greener alternative

sufficient for 5 preparations

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Synonym(s):
HP gigaprep kit, endo free gigaprep kit, endotoxin free giga kit, endotoxin free plasmid kit, fast gigaprep kit, fast plasmid isolation, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, ultrafast plasmid isolation, Gen Elute, GenElute HP Plasmid Kit
NACRES:
NA.52

usage

sufficient for 5 preparations

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

technique(s)

DNA extraction: suitable

greener alternative category

storage temp.

15-25°C

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This Item
NA0600NA0400NA9604
usage

sufficient for 5 preparations

usage

 kit sufficient for 5 preparations

usage

sufficient for 10 preparations

usage

sufficient for 4 96-well plate purifications

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

-

greener alternative category

Aligned,

greener alternative category

, Aligned

greener alternative category

Aligned,

greener alternative category

-

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

The GenElute HP Select Plasmid Gigaprep Kit offers a simple, rapid, and cost effective method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. Up to 15 mg of high copy plasmid DNA with ≤0.1 endotoxin unit/μg plasmid DNA can be recovered from E. coli cultures in 1.2-2.5 L Luria Broth (LB) medium or 600 mL-1.2 L of Terrific Broth (TB) medium in less than 2 hours after harvesting the cells. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis. The lysate is cleared by filtration followed by the addition of a binding solution optimized for endotoxin-free plasmid preparations. The plasmid DNA is then captured onto a silica membrane in the presence of high salts while endotoxins are prevented from adsorbing to the membrane. Contaminants are removed with three wash steps. Finally, the bound DNA is eluted in endotoxin-free water.

The DNA is ready for immediate use in applications such as restriction digest, cloning, sequencing, PCR, and transfection of endotoxin-sensitive cell lines.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

GenElute® HP Select Plasmid Gigaprep Kit has been used to purify plasmids.
High-quality, endotoxin-free DNA is ready for immediate use for the most demanding applications including transfection with endotoxin-sensitive cells and gene therapy research.

Features and Benefits

  • Up to 15 mg with endotoxin levels of ≤ 0.1 EU/μg
  • Only 90 minutes from pelleted cells to purified plasmid
  • Vacuum format with no ethanol precipitation
  • Can purify low-, medium-, and high-copy plasmid DNA

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

Gabitril is a registered trademark of Abbott Laboratories Corp.
GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS

  • G3671GenElute HP Pro Gigaprep Binding ColumnSDS

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3

target_organs

Central nervous system, Lungs

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

77.0 °F - closed cup

flash_point_c

25 °C - closed cup


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Plasmid DNA-coding p62 as a bone effective anti-inflammatory/anabolic agent.
Sabbieti MG
Oncotarget, 6(6), 3590-3599 (2015)
V D Boppana et al.
Parasite immunology, 31(6), 287-295 (2009-06-06)
Mosquitoes represent the most important vector for transmitting pathogens that cause human disease. Central to pathogen transmission is the ability to divert the host immune system away from Th1 and towards Th2 responsiveness. Identification of the mosquito factor(s) critical for

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