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NA2100

Sigma-Aldrich

GenElute Bacterial Genomic DNA Kits

sufficient for 10 purifications

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Synonym(s):
Bacterial Genomic DNA, Gen Elute
NACRES:
NA.55

usage

sufficient for 10 purifications

Quality Level

storage temp.

15-25°C

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This Item
NA2120NA2110G1N10

NA2110

NA2100, NA2110

NA2100, NA2120

G1N350, G1N70, G1N9602, G1N9604

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in conjunction with the optional lysozyme (L4919) to effectively lyse the thick peptidoglycan cell walls. A Gram-Positive Lysis Solution is provided as a diluent for preparing the lysozyme stock solutions.

The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Application

The purified bacterial genomic DNA is ready for downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • cloning

Features and Benefits

  • Starting material: Up to 1.5 mL of culture
  • Expected yield: Up to 20 μg
  • Elution volume: 400 μl
  • Time required: 70 - 120 min
  • A260/A280 ratio: 1.6 - 1.9
  • No phenol, chloroform, or ethanol precipitation required

Principle

The bacteria are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane into a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution.

The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS

  • W0263Wash Solution 1SDS

  • C2112Column Preparation SolutionSDS

  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biologySDS

  • R6148RNase A solutionSDS

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2

Storage Class

3 - Flammable liquids

wgk_germany

WGK 2


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Cecilia A Silva et al.
Infection and immunity, 80(2), 839-849 (2011-11-16)
Salmonella enterica serovar Enteritidis causes a systemic, typhoid-like infection in newly hatched poultry and mice. In the present study, a library of 54,000 transposon mutants of S. Enteritidis phage type 4 (PT4) strain P125109 was screened for mutants deficient in
Ivan M Mukisa et al.
International journal of food microbiology, 160(1), 1-10 (2012-11-13)
Obushera includes four fermented cereal beverages from Uganda namely: Obutoko, Enturire, Ekitiribita and Obuteire, whose microbial diversity has not hitherto been fully investigated. Knowledge of the microbial diversity and dynamics in these products is crucial for understanding their safety and
Pierre Stallforth et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(36), 14528-14533 (2013-07-31)
Stable multipartite mutualistic associations require that all partners benefit. We show that a single mutational step is sufficient to turn a symbiotic bacterium from an inedible but host-beneficial secondary metabolite producer into a host food source. The bacteria's host is
Lizbeth Sayavedra et al.
Environmental microbiology, 23(6), 3164-3181 (2021-04-21)
Sulfate-reducing bacteria (SRB) are widespread in human guts, yet their expansion has been linked to colonic diseases. We report the isolation, sequencing and physiological characterization of strain QI0027T , a novel SRB species belonging to the class Desulfovibrionia. Metagenomic sequencing
Ramon Hochstrasser et al.
Applied and environmental microbiology, 88(5), e0237021-e0237021 (2022-01-06)
Legionella species are facultative intracellular pathogens that cause a life-threatening pneumonia termed Legionnaires' disease. Legionella pneumophila employs the Lqs-LvbR (Legionella quorum sensing-Legionella virulence and biofilm regulator) network to regulate virulence and motility, but its role for growth in media is

Protocols

GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service