The formulation of the lysis solution in this kit is proprietary. However, it does not contain Trizol.
NA2110
GenElute™ Bacterial Genomic DNA Kits
sufficient for 70 purifications
Synonym(s):
Bacterial Genomic DNA, bacterial DNA prep, bacterial DNA purification kit, bacterial gDNA extraction kit, bacterial gDNA isolation kit, bacterial gDNA purification kit, bacterial genomic DNA extraction kit, bacterial genomic DNA isolation kit, bacterial genomic DNA purification kit, Gen Elute
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$242.00
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About This Item
NACRES:
NA.55
UNSPSC Code:
41105501
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Product Name
GenElute™ Bacterial Genomic DNA Kits, sufficient for 70 purifications
usage
sufficient for 70 purifications
technique(s)
DNA purification: suitable
Quality Level
test parameters
: 70—120 min kit run time
sample volume: 1.5 mL bacterial culture
storage temp.
15-25°C
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Related Categories
1 of 4
This Item | NA2120 | G1N350 | NA2100 |
|---|---|---|---|
| usage sufficient for 70 purifications | usage sufficient for 350 purifications | usage sufficient for 350 purifications | usage sufficient for 10 purifications |
| technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) - |
NA2100 | NA2100, NA2110 | G1N10, G1N70, G1N9602, G1N9604 | NA2110, NA2120 |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Application
The purified bacterial genomic DNA is ready for downstream applications such as:
- restriction endonuclease digestions
- PCR
- Southern blots
- cloning
Biochem/physiol Actions
The bacteria are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane into a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution.
The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.
The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.
Features and Benefits
- Starting material: Up to 1.5 mL of culture
- Expected yield: Up to 20 μg
- Elution volume: 400 μl
- Time required: 70 - 120 min
- A260/A280 ratio: 1.6 - 1.9
- No phenol, chloroform, or ethanol precipitation required
- Typical DNA yields of 15 μg - 20 μg
- Protocols provided for Gram + and Gram - bacteria
- High quality genomic DNA in less than 2 hours
- Purified DNA has an A260/A280 ratio between 1.6 and 1.9
General description
The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in conjunction with the optional lysozyme (L4919) to effectively lyse the thick peptidoglycan cell walls. A Gram-Positive Lysis Solution is provided as a diluent for preparing the lysozyme stock solutions.
The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.
The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.
Other Notes
For additional information, please see www.sigma-aldrich.com/genomicdna.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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signalword
Danger
target_organs
Central nervous system, Respiratory system
Storage Class
3 - Flammable liquids
flash_point_f
77.0 °F - closed cup
flash_point_c
25 °C - closed cup
wgk
WGK 3
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
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Articles
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
Protocols
GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.
Related Content
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
Resumen de las técnicas habituales y las aplicaciones posteriores de la extracción y purificación del ADN genómico, el ADN plasmídico y el ARN total de células, tejidos, sangre, virus y otros tipos de muestra.
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Where can I find the compositions of the reagents in the bacterial genomic DNA isolation kit? Does Lysis C contain trizol?
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