NA2120

Sigma-Aldrich

GenElute Bacterial Genomic DNA Kits

sufficient for 350 purifications

Synonym(s):
Bacterial Genomic DNA, Gen Elute
NACRES:
NA.55

Quality Level

usage

sufficient for 350 purifications

storage temp.

room temp

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General description

The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in conjunction with the optional lysozyme (L4919) to effectively lyse the thick peptidoglycan cell walls. A Gram-Positive Lysis Solution is provided as a diluent for preparing the lysozyme stock solutions.

The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Application

The purified bacterial genomic DNA is ready for downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • cloning

GenElute Bacterial Genomic DNA Kits has been used:
  • to isolate DNA
  • to prepare DNA from E. coli strain
  • to extract genomic DNA

The purified bacterial genomic DNA is ready for downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • cloning

Features and Benefits

  • Starting material: Up to 1.5 mL of culture
  • Expected yield: Up to 20 μg
  • Elution volume: 400 μl
  • Time required: 70 - 120 min
  • A260/A280 ratio: 1.6 - 1.9
  • No phenol, chloroform, or ethanol precipitation required

Principle

The bacteria are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane into a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution.

The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS

  • W0263Wash Solution 1

  • C2112Column Preparation Solution

  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology

  • R6148RNase A solution

Signal Word

Danger

hazcat

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system

storage_class_code

3 - Flammable liquids

WGK Germany

WGK 3

Flash Point(F)

77.0 °F - closed cup

Flash Point(C)

25 °C - closed cup

Certificate of Analysis

Certificate of Origin

Molecular characterization of serogroup 19 Streptococcus pneumoniae in the Czech Republic in the post-vaccine era
VZemlivckova H, et al.
Journal of Medical Microbiology, 67(7), 1003-1003 (2018)
Saliya Gurusinghe et al.
Molecules (Basel, Switzerland), 24(10) (2019-05-24)
Small molecule discovery has benefitted from the development of technologies that have aided in the culture and identification of soil microorganisms and the subsequent analysis of their respective metabolomes. We report herein on the use of both culture dependent and...
Modulation of Global Transcriptional Regulatory Networks as a Strategy for Increasing Kanamycin Resistance of the Translational Elongation Factor-G Mutants in Escherichia coli
Mogre A, et al.
G3 (Bethesda, Md.), g3-300284 (2017)
Alexander W Eastman et al.
Genome announcements, 2(1) (2014-01-25)
Here we report the complete genome sequence of the bacterium Paenibacillus polymyxa CR1 (accession no. CP006941), which consists of one circular chromosome of 6,024,666 bp with 5,283 coding sequences (CDS), 87 tRNAs, and 12 rRNA operons. Data presented will allow for...
Isabella Santi et al.
mBio, 12(1) (2021-02-11)
The widespread use of antibiotics promotes the evolution and dissemination of resistance and tolerance mechanisms. To assess the relevance of tolerance and its implications for resistance development, we used in vitro evolution and analyzed the inpatient microevolution of Pseudomonas aeruginosa...
Protocols
GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.
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