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NUC101

Sigma-Aldrich

Nuclei Isolation Kit: Nuclei EZ Prep

sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)

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Synonym(s):
Rapid mammalian nuclei isolation kit
NACRES:
NA.32

usage

sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)

shelf life

1 yr at 2‑8 °C

packaging

pkg of 1 kit

application(s)

cell analysis

foreign activity

nuclease and protease, free

shipped in

wet ice

storage temp.

2-8°C

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shelf life

1 yr at 2‑8 °C

shelf life

-

shelf life

-

shelf life

-

packaging

pkg of 1 kit

packaging

pkg of 1 kit

packaging

-

packaging

pkg of 48 units (bulk packaging)

application(s)

cell analysis

application(s)

cell analysis

application(s)

food and beverages

application(s)

bioburden testing
food and beverages
water monitoring

foreign activity

nuclease and protease, free

foreign activity

nuclease and protease, free

foreign activity

-

foreign activity

-

shipped in

wet ice

shipped in

wet ice

shipped in

ambient

shipped in

-

General description

The Nuclei EZ Prep Kit was developed for the rapid isolation of nuclei from mammalian cells. The kit provides a high yield of nuclei from commonly used cell types.

Application

Suitable as a source of nuclear components, to produce nuclei for in vitro apoptosis assays, and for functional studies.

Biochem/physiol Actions

The protocol provides a high yield of nuclei from commonly used mammalian cells, including both adherent (e.g., HEK293 and COS7) and non-adherent (e.g., Jurkat and HFN7.1) cell lines and peripheral blood mononuclear cells (PBMCs). The preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

Other Notes

All procedures should be carried out on ice or 2-8°C.

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Organelle Isolation

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.

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