O2260

Mouse Osteopontin

recombinant, expressed in NSO cells, powder, suitable for cell culture

• Synonym(s): Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD03100698
• Biological source: mouse
• Form: lyophilized powder
• Technique(s): cell culture | mammalian: suitable
• Mol wt: 30 kDa by SDS-PAGE (reducing), 65 kDa by SDS-PAGE (reducing) (doublet)
• Assay: >95% (SDS-PAGE)

Properties

• Product Name: Osteopontin from mouse, >95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture
• biological source: mouse
• recombinant: expressed in NSO cells
• sterility: sterile
• assay: >95% (SDS-PAGE)
• form: lyophilized powder
• mol wt: 30 kDa by SDS-PAGE (reducing), 65 kDa by SDS-PAGE (reducing) (doublet)
• packaging: pkg of 50 μg
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P10923
• shipped in: dry ice
• storage temp.: −20°C
• Quality Level: 100
• Gene Information: mouse ... Spp1(20750)

Description

Application

Osteopontin from mouse has been used:

• to study its role in a mouse model of preterm brain injury[1]
• as a positive control to analyze the relationship between the vascular calcification process and osteopontin (OPN) overexpression in the neointima of atherosclerotic plaques[2]
• to study its interaction on the crystal growth process of hydroxyapatite in agarose hydrogels[3]

Biochem/physiol Actions

Osteopontin (OPN) is a secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity.[4] Human, mouse, rat, pig and bovine OPN share 40% amino acid sequence identity.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to mediate 293 cell adhesion.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable