The concentration of BSE in this product is approximately 100 mg/mL in DPBS. The lot-specific concentration is reported in the Certificate of Analysis as "Protein by Biuret". Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/o3008#product-documentation
O3008
Oleic Acid-Albumin from bovine serum
liquid, sterile-filtered, BioReagent, suitable for cell culture
Oleic Acid-Albumin from bovine serum
Synonym(s):
Oleic acid-BSA complex
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| Size/SKU | Availability | Price |
|---|---|---|
5 mL | Check Cart for Availability | $41.70 |
About This Item
Biological source:
bovine
Form:
liquid
Technique(s):
cell culture | mammalian: suitable
Impurities:
endotoxin, tested
Concentration:
100 mg/mL BSA in DPBS, 90-120 mg/mL protein (biuret)
biological source
bovine
Quality Segment
sterility
sterile-filtered
product line
BioReagent
form
liquid
composition
Oleic acid, 2 mol/mol albumin
concentration
100 mg/mL BSA in DPBS, 90-120 mg/mL protein (biuret)
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
storage temp.
2-8°C
Application
Oleic Acid-Albumin from bovine serum has been used: as a component of the Dulbecco′s modified Eagle′s medium (DMEM) to study adipogenic differentiation in chicken embryonic fibroblast cell lines[1], to study its effects on benign prostate epithelial cells[2] as a component of the cell culture medium to quantify triglycerides in human epithelial colorectal adenocarcinoma cells[3]
Biochem/physiol Actions
Fatty Acid-Albumin - complexes are used as alternative sources of lipids in the development of serum-free media. Fatty acids bind to serum proteins in high proportions. Serum proteins may help in the release of beneficial fatty acids and bind those that are inhibitory. Oleic acid bound to bovine serum albumin (BSA) shows a positive effect on the growth of various cells.
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This Item | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| biological source bovine | biological source - | biological source bovine | biological source bovine |
| form liquid | form liquid | form lyophilized powder | form lyophilized powder |
| impurities endotoxin, tested | impurities ≤400 EU/mL endotoxin | impurities essentially globulin free | impurities ≤20 ppm Pb, ≤3 EU/mg endotoxin |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| sterility sterile-filtered | sterility sterile-filtered | sterility - | sterility - |
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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What is the bsa concentration in the solution?
1 answer-
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Is it suitable to store this reagent in -20C in aliquots? We received our order recently and the shipping box has a large label of "Do not Freeze" I was inquiring if there was a decrease in concentration if frozen?
1 answer-
To effectively preserve the integrity of this product, it should be stored at the recommended temperature and protected from light. The stability and optimal performance may be impacted by the freeze/thaw process. This product should be stored at 2 to 8 C.
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How is the binding of oleic acid to BSA is confirmed?
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Many fatty acids have a natural affinity to some plasma proteins. In this case BSA serves as a carrier or transporter of the insoluble oleic acid. The product is tested for protein and oleic acid content, only. No additional testing is performed to confirm the complex formation. See the following publications for further information:
https://pubmed.ncbi.nlm.nih.gov/236351/
https://pubmed.ncbi.nlm.nih.gov/19745557/#:~:text=Albumin%20acts%20as%20main%20fatty,a%20several%20orders%20of%20magnitudeHelpful?
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What is the concentration of Oleic Acid in this product in Molarity (M) or milimolarity (mM)? I need this to be able to follow a protocol
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Oleic acid has a molecular mass of 282.47 g/mol. In order to find the concentration of oleic acid in a specific lot, use the Oleic acid concentration on the lot-specific certificate of analysis (COA).
The equation used to find the concentration is as follows: (molar mass of Oleic acid) x (concentration of Oleic acid from COA), this will result in units of liter/mole which can be multiplied by the reciprocal, resulting in units of Molarity (mole/liter).
For example, for lot number SLCM9550, the Oleic acid concentration is reported as 940 ug/mL.
282.47 g/mol / 0.94 g/L =300.5 L/mol
1/300.5= 0.003327 M (mol/liter) = 3.33 mM Oleic AcidHelpful?
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Through what method is oleic acid complexed to BSA?
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Unfortunately, the method of preparation considered proprietary.
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