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O4264

Sigma-Aldrich

Osteopontin human

recombinant, expressed in NSO cells, ≥95% (SDS-PAGE), lyophilized powder, suitable for cell culture

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Synonym(s):
Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)
MDL number:
NACRES:
NA.32

biological source

human

Quality Level

recombinant

expressed in NSO cells

sterility

sterile

assay

≥95% (SDS-PAGE)

form

lyophilized powder

mol wt

60-65 kDa by SDS-PAGE (reducing)

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

shipped in

ambient

storage temp.

−20°C

Gene Information

human ... SPP1(6696)

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This Item
SRP3131H9661O2260
Sigma-Aldrich

O4264

Osteopontin human

Sigma-Aldrich

SRP3131

Osteopontin human

Sigma-Aldrich

H9661

Hepatocyte Growth Factor human

Sigma-Aldrich

O2260

Osteopontin from mouse

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile

sterility

-

sterility

-

sterility

sterile

assay

≥95% (SDS-PAGE)

assay

≥97% (HPLC), ≥97% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

>95% (SDS-PAGE)

Quality Level

100

Quality Level

-

Quality Level

200

Quality Level

100

recombinant

expressed in NSO cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in NSO cells

recombinant

expressed in NSO cells

General description

Secreted phosphoprotein 1 (SPP1) or osteopontin is a secreted cytokine. This phosphorylated acidic glycoprotein is expressed in various cells and tissues. It is synthesized by osteoblasts. The gene encoding SPP1 is localized on human chromosome 4q22.1.

Application

Anti-Osteopontin antibody produced in rabbit has been used for ELISA.

Biochem/physiol Actions

A secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig, and bovine OPN share 40% amino acid sequence identity. Secreted phosphoprotein 1 (SPP1) or osteopontin modulates inflammatory responses and glucose metabolism. It is a chemotactic factor and has a role in immune cell recruitment. The protein is also associated with cell growth, migration and differentiation. SPP1 attaches osteoclasts to the bone matrix. It is also involved in atherosclerotic process and bone mineralization. It is a marker of many cancers.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to mediate 293 cell adhesion.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Osteopontin Fragments with Intact Thrombin-Sensitive Site Circulate in Cervical Cancer Patients
Danny TM
PLoS ONE (2016)
Comparison of Plasma Osteopontin Levels between Patients with Borderline Ovarian Tumours and Serous Ovarian Carcinoma.
Zivny JH
Folia Biologica (Praha.) (2016)
Expanded comparative mapping between man
and rabbit and detection of a new conserved
segment between HSA22 and OCU4
C Chantry
Cytogenetic and genome research (2005)
Polycystic ovary syndrome is associated with increased osteopontin levels
European Journal of Endocrinology (2015)
Utility of Plasma Osteopontin Levels in Management of Community-Acquired Pneumonia
Jer-Hwa Chang
International Journal of Medical Sciences (2016)

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