O4878

Oxaloacetate Decarboxylase from Pseudomonas sp.

Name: Oxaloacetate Decarboxylase from <I>Pseudomonas</I> sp.
Brand: SIGMA
Price: 274 USD
Availability: InStock

lyophilized powder, ≥100 units/mg solid

Synonym(s):

OAD

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About This Item

CAS Number:

9024-98-0

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

4.1.1.3 (BRENDA, IUBMB)

MDL number:

MFCD00131775

Specific activity:

≥100 units/mg solid

Key Documents

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Product Name

Oxaloacetate Decarboxylase from Pseudomonas sp., lyophilized powder, ≥100 units/mg solid

form

lyophilized powder

specific activity

≥100 units/mg solid

storage temp.

−20°C

Quality Level

200

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This Item	C1403	A3352	L9518
Sigma-Aldrich O4878 Oxaloacetate Decarboxylase from Pseudomonas sp.	Sigma-Aldrich C1403 Cholesterol Esterase from Pseudomonas sp.	Sigma-Aldrich A3352 Acyl-coenzyme A Synthetase from Pseudomonas sp.	Sigma-Aldrich L9518 Lipase from Pseudomonas sp.
specific activity ≥100 units/mg solid	specific activity ≥200,000 units/g protein	specific activity ≥2 units/mg protein	specific activity ≥15 units/mg solid
form lyophilized powder	form lyophilized powder	form powder	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

Application

Oxaloacetate Decarboxylase from Pseudomonas sp. has been used in the digestion of the low molecular weight (LMW) human milk fraction (5kF fraction)[1] and as a positive control for deciphering C. thermocellum oxaloacetate decarboxylase activity.[2]

Oxaloacetate decarboxylase has been used in a study to assess turnover and accessibility of a reentrant loop of the Na(+)-glutamate transporter GltS. [3] It has also been used in a study to investigate fermentation and metabolic characteristics of Gluconacetobacter oboediens for different carbon sources. [4]

Biochem/physiol Actions

Oxaloacetate Decarboxylase catalyzes the decarboxylation of oxaloacetate and requires manganese and magnesium for its activity.[5] It is associated with a wide vareity of Gram-negative bacteria.[6]

General description

Oxaloacetate decarboxylase or OAD functions as a Na pump in anaerobic bacteria. It is a membrane protein consisting of three subunits, α, β and γ with the α subunit containing the carboxylase activity. [7]

Other Notes

One unit will convert 1.0 μmole of oxalacetate to pyruvate and CO₂ per min at pH 8.0 at 25 °C.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000500371

0000472134

0000402224

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Characterization of an oxaloacetate decarboxylase that belongs to the malic enzyme family.

Pablo D Sender et al.

FEBS letters, 570(1-3), 217-222 (2004-07-15)

The citM gene from Lactococcus lactis CRL264 was demonstrated to encode for an oxaloacetate decarboxylase. The enzyme exhibits high levels of similarity to malic enzymes (MEs) from other organisms. CitM was expressed in Escherichia coli, purified and its oxaloacetate decarboxylase

Identification of a domain in the alpha-subunit of the oxaloacetate decarboxylase Na+ pump that accomplishes complex formation with the gamma-subunit.

Pius Dahinden et al.

The FEBS journal, 272(3), 846-855 (2005-01-27)

The oxaloacetate decarboxylase Na+ pumps OAD-1 and OAD-2 of Vibrio cholerae are composed of a peripheral alpha-subunit associated with two integral membrane-bound subunits (beta and gamma). The alpha-subunit contains the carboxyltransferase domain in its N-terminal portion and the biotin-binding domain

Turnover and accessibility of a reentrant loop of the Na+-glutamate transporter GltS are modulated by the central cytoplasmic loop.

Tomas Krupnik et al.

Molecular membrane biology, 28(7-8), 462-472 (2011-10-15)

GltS of Escherichia coli is a secondary transporter that catalyzes Na+-glutamate symport. The structural model of GltS shows two homologous domains with inverted membrane topology that are connected by a central loop that resides in the cytoplasm. Each domain contains

Oxalacetic carboxylase of Micrococcus lysodeikticus.

Herbert, D.

Methods in Enzymology, 1, 753-757 (1955)

Quaternary structure of the oxaloacetate decarboxylase membrane complex and mechanistic relationships to pyruvate carboxylases.

Monica Balsera et al.

The Journal of biological chemistry, 286(11), 9457-9467 (2011-01-07)

The oxaloacetate decarboxylase primary Na(+) pump (OAD) is an essential membrane protein complex that functions in the citrate fermentation pathway of some pathogenic bacteria under anaerobic conditions. OAD contains three different subunits: Oad-α, a biotinylated extrinsic protein that catalyzes the

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Oxaloacetate Decarboxylase from Pseudomonas sp.