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O4886

Sigma-Aldrich

Monoclonal Anti-Opsin antibody produced in mouse

clone RET-P1, ascites fluid

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MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

RET-P1, monoclonal

mol wt

antigen 39 kDa by immunoblotting (IB of rat retina produces closely spaced doublet)

contains

15 mM sodium azide

species reactivity

goldfish, mouse, tiger salamander, rat, amphibian, quail, dove, duck, rabbit, bovine, human, turtle

technique(s)

electron microscopy: suitable
immunocytochemistry: suitable using cultured cells
immunohistochemistry (frozen sections): 1:10,000 using frozen sections of rat eye
indirect ELISA: suitable
radioimmunoassay: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RHO(6010)
mouse ... Rho(212541)
rat ... Rho(24717)

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SAB4200762R5403R129
conjugate

unconjugated

conjugate

-

conjugate

unconjugated

conjugate

unconjugated

Gene Information

human ... RHO(6010)
mouse ... Rho(212541)
rat ... Rho(24717)

Gene Information

rat ... Rho(24717)

Gene Information

human ... RHO(6010)
rat ... Rho(24717)

Gene Information

human ... RYR1(6261), RYR2(6262)
mouse ... Ryr1(20190), Ryr2(20191)
rat ... Ryr1(114207), Ryr2(84025)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

ascites fluid

species reactivity

goldfish, mouse, tiger salamander, rat, amphibian, quail, dove, duck, rabbit, bovine, human, turtle

species reactivity

avian, bovine, fish, mouse, rat, human, amphibian, turtle, rabbit

species reactivity

human, rat, bovine

species reactivity

human, rat, rabbit, canine, mink, bovine, frog, fish, primate, chicken, mouse, sheep

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General description

Mouse monoclonal clone RET-P1 anti-Opsin antibody recognizes an epitope located in amino acid residues 4-10 at the N-terminus of the rhodopsin molecule. In immunoblotting of rat retina, the antibody labels a closely spaced doublet of 39 kDa and less intense bands of 78 and 115 kDa, representing rhodopsin monomer and aggregates. Higher M.W. bands are stained in pituitary (251 and 288 kDa) and in hypothalamus (269 and 331 kDa) in addition to several lower M.W. bands. The product specifically labels the cell bodies, outer and inner segments (rods but not cones) of rat photoreceptor surface. It reacts with bovine, duck, amphibian, goldfish, rabbit, rat, turtle, tiger salamander, mouse, dove, quail, and human rhodopsin/opsin.
Opsin belongs to rhodopsin receptor. The gene is located on human chromosome 3q22.1. Opsin gene is mainly expressed in rod cells.
Photoreceptors are responsible for the initial step in visual processing, converting the signal of photon absorption into synaptic transmission. The visual pigment in vertebrate rods responsible for the absorption of light quanta is rhodopsin (also called opsin). Rhodopsin comprises >95% of the rod outer segments (ROS) intrinsic membrane protein and is a glycoprotein possessing two asparagine-linked oligosaccharide groups at amino acid residues 2 and 15 of the N-terminus. Light is absorbed by rhodopsin and the subsequent conformational change leads to the activation of a cyclic GMP phosphodiesterase through a specific G-protein intermediate, transducin.

Immunogen

rat retinal membranes.

Application

Monoclonal Anti-Opsin antibody produced in mouse has been used in immunofluorescence and histology.
Mouse monoclonal clone RET-P1 anti-Opsin antibody is used to tag opsin/rhodopsin for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as immunoblotting, immunocytochemistry of cultured cells, immunohistochemistry (paraformaldehyde/glutaraldehyde-fixed, paraformaldehyde perfusion-fixed, frozen sections), immunoelectron microscopy, ELISA, competitive ELISA, and solid phase RIA. It is used as a probe to determine the presence and roles of opsin/rhodopsin in studies of rhodopsin location, functional properties and molecular mechanisms governing rod photoreceptor differentiation.

Biochem/physiol Actions

Mutations in rhodopsin gene are linked to retinitis pigmentosa (RP) and congenital night blindness.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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