O8137

Osteoprotegerin/Fc Chimera from mouse

>95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

• Synonym(s): OCIF, OPG
• NACRES: NA.32
• PubChem Substance ID: 24898055
• UNSPSC Code: 12352202
• MDL number: MFCD01867131

Properties

• InChI key: XXUPLYBCNPLTIW-UHFFFAOYSA-N
• SMILES string: CCCCCCCCCCC#CCCCCCC(O)=O
• InChI: 1S/C18H32O2/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18(19)20/h2-10,13-17H2,1H3,(H,19,20)
• biological source: mouse
• recombinant: expressed in NSO cells
• assay: >95% (SDS-PAGE)
• form: lyophilized powder
• potency: 25-50 ng/mL ED₅₀
• mol wt: 100 kDa by SDS-PAGE (reducing), calculated mol wt 70.9 kDa
• packaging: pkg of 100 μg
• impurities: endotoxin, tested
• UniProt accession no.: O08712
• storage temp.: −20°C
• Quality Level: 200
• Gene Information: mouse ... Tnfrsf11b(18383)

Description

General description

Osteoprotegerin (OPG) is a member of the TNFR (tumor necrosis factor receptor) superfamily that can act as a decoy receptor for RANKL (receptor activator of nuclear factor κ-B ligand) and TRAIL (tumor necrosis factor-related apoptosis-inducing ligand).[1][2] The primary structure of OPG protein consists of seven distinct domains, four of which corresponds to the extracellular cysteine-rich domains of TNFR proteins and constitutes the soluble OPG. OPG is expressed in a wide variety of tissues including adult heart, lung, kidney and liver. It is secreted both as a monomeric and a dimeric protein.[3]

Biochem/physiol Actions

Binding of soluble osteoprotegerin (OPG) to sRANKL (soluble receptor activator of nuclear factor κ-B ligand) inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage.[1] Changes in the level of serum OPG are associated with atherosclerosis and cardiovascular disorders.[2] It might also be associated with malignancy and plays a regulatory role in multiple myeloma, prostate, breast, bladder and gastric carcinoma.[4] OPG plays a vital role in bone metabolism and is involved in inhibition of ovariectomy-associated bone loss in rats.[3][4]

OPG is a soluble secreted member of the tumor necrosis factor receptor superfamily that lacks any apparent cell-association motifs. It regulates the formation of osteoclasts and is involved in the regulation of bone resorption and remodeling. Human and mouse OPG share approximately 84% and 94% amino acid sequence identity, respectively, with rat OPG.

Soluble secreted member of the tumor necrosis factor receptor superfamily that lacks any apparent cell-association motifs; regulates the formation of osteoclasts and is involved in the regulation of bone resorption and remodeling.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.

Analysis Note

The biological activity is measured by its ability to neutralize apoptosis of mouse L-929 cells treated with 50 ng/ml recombinant human TRAIL.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable