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O9515

Sigma-Aldrich

Prolyl oligopeptidase

recombinant, expressed in E. coli

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Synonym(s):
Prolyl endopeptidase
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

assay

≥90% (SDS-PAGE)

form

solution

specific activity

≥10 unit/μg protein

mol wt

81.6 kDa

shipped in

dry ice

storage temp.

−70°C

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This Item
K4519164599SAB1401302
vibrant-m

O9515

Prolyl oligopeptidase

vibrant-m

K4519

Keratinase

vibrant-m

164599

Aminopeptidase N, Rat

specific activity

≥10 unit/μg protein

specific activity

300-1000 units/mg protein

specific activity

≥100 units/mg protein

specific activity

-

shipped in

dry ice

shipped in

-

shipped in

dry ice

shipped in

dry ice

storage temp.

−70°C

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−20°C

form

solution

form

lyophilized powder

form

liquid

form

buffered aqueous solution

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

General description

Prolyl oligopeptidase (PO) is mapped to human chromosome 6q22. It comprises of a N-terminal β-propeller domain and C-terminal α/β hydrolase catalytic domain. PO is highly expressed in the neuronal cytoplasm and its expression increases with age.
Prolyl oligopeptidase is a cytosolic serine peptidase which cleaves peptide bonds at the C′ terminal side of prolines. It is only capable of processing peptides containing no more than 30 amino acids due to the unique β-propeller region that regulates access to the active site.

Application

Prolyl oligopeptidase has been used in a study to assess the mechanism of lithium ion action. It has also been used in a study to investigate its distribution in human tissue and body fluids.
Prolyl oligopeptidase has been used in the prolyl oligopeptidase inhibitory activity assay in lyophilized protein hydrolysate samples and Schistosoma mansoni samples.

Biochem/physiol Actions

Prolyl oligopeptidase (PO) regulates physiological processes and is crucial for the generation of active hormone and peptide fragments from their precursors. It shows elevated levels in psychiatric disorders and Alzheimer′s patients. Altered levels of PO is observed in patients with bipolar disorder. High expression of PO may promote metastasis of malignant ovarian and colorectal tumors.

Unit Definition

One unit will hydrolyze 1.0 picomole of Ala-Pro-aminomethylcoumarin per minute at pH 7.4 at 25 °C.

Physical form

Supplied as a solution in 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, 3 mM DTT and variable amounts of imidazole.

Preparation Note

N-terminal GST-tagged 81.6 kDa full-length protein

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Mechanism of Action of Prolyl Oligopeptidase (PREP) in Degenerative Brain Diseases: Has Peptidase Activity Only a Modulatory Role on the Interactions of PREP with Proteins?
Mannisto PT, et al.
Frontiers in Aging Neuroscience, 9(3), 27-27 (2017)
R S Williams et al.
The EMBO journal, 18(10), 2734-2745 (1999-05-18)
The therapeutic properties of lithium ions (Li+) are well known; however, the mechanism of their action remains unclear. To investigate this problem, we have isolated Li+-resistant mutants from Dictyostelium. Here, we describe the analysis of one of these mutants. This
Structure-function properties of prolyl oligopeptidase family enzymes
Rea D and Fulop V
Cell Biochemistry and Biophysics, 44(3), 349-365 (2006)
Prolyl oligopeptidase from the blood fluke Schistosoma mansoni: from functional analysis to anti-schistosomal inhibitors
Fajtova P, et al.
PLoS Neglected Tropical Diseases, 9(6), e0003827-e0003827 (2015)
Prolyl oligopeptidase and bipolar disorder
Williams, Robin SB
Clinical neuroscience research, 4(3-4), 233-242 (2004)

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