O9755

Oil Red O

BioReagent, suitable for electrophoresis

• Synonym(s): 1-([4-(Xylylazo)xylyl]azo)-2-naphthol, 1-[2,5-Dimethyl-4-(2,5-dimethylphenylazo)phenylazo]-2-naphthol, Solvent Red 27
• Empirical Formula (Hill Notation): C₂₆H₂₄N₄O
• CAS Number: 1320-06-5
• Molecular Weight: 408.49
• Colour Index Number: 26125
• EC Number: 215-295-3
• MDL number: MFCD00003898
• PubChem Substance ID: 24898072
• NACRES: NA.32

Properties

• product line: BioReagent
• Quality Level: 100
• form: powder
• technique(s): electrophoresis: suitable
• mp: 120 °C (dec.) (lit.)
• λmax: 359 nm; 518 nm (2nd)
• suitability: suitable for electrophoresis
• SMILES string: Cc1ccc(C)c(c1)\N=N\c2cc(C)c(cc2C)\N=N/c3c(O)ccc4ccccc34
• InChI: 1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,31H,1-4H3
• InChI key: NPGIHFRTRXVWOY-UHFFFAOYSA-N

Description

Application

Oil Red O stain is suitable as a lipid/lipoprotein stain on cellulose acetate. A 0.2% stock solution is prepared in methanol. The stock solution (35 ml) is diluted with 10 mL of 1 M NaOH immediately before staining. The dye begins precipitating during staining. After staining (1 hr), the membrane is destained in methanol:glycerol (1:3). Often cited for use with agarose

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves