Documents
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packaging
vial of 20 μL
Quality Level
concentration
500 ng/μL
application(s)
genome mapping
genomic analysis
shipped in
dry ice
storage temp.
−70°C
Related Categories
1 of 4
This Item | ORFBFPH | ORFGFPV | ORFGFP |
|---|---|---|---|
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. −70°C | storage temp. −70°C | storage temp. −70°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| packaging vial of 20 μL | packaging vial of 200 μL | packaging vial of 200 μL | packaging vial of 20 μL |
| application(s) genome mapping | application(s) genome mapping | application(s) genome mapping | application(s) genome mapping |
General description
This positive control can be used for measuring transduction efficiency and optimizing ORF delivery in cell lines that do not survive any puromycin exposure. The BFP Control contains a gene encoding BFP driven by the EF1a promoter. This control provides fast visual confirmation of successful transduction. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively.
In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The MISSION TRC3 ORF BFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Application
Legal Information
related product
Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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