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ORFGFP

Sigma-Aldrich

MISSION® TRC3 ORF GFP DNA Control

NACRES:
NA.51

packaging

vial of 20 μL

Quality Level

concentration

500 ng/μL

application(s)

genome mapping
genomic analysis

shipped in

dry ice

storage temp.

−20°C

General description

The MISSION TRC3 ORF GFP Control Vector is a lentivirus plasmid vector. The vector contains an ORF insert expressing TurboGFP, and is useful as a positive (or negative control) in experiments using the MISSION TRC3 LentiORF Library Collection in arrayed or pooled format, including our latest Whole Genome LentiORF Pool. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.

This positive control can be used for measuring transduction efficiency and optimizing ORF delivery in cell lines that do not survive any puromycin exposure. The GFP Control contains a gene encoding TurboGFP driven by the EF1a promoter. This control provides fast visual confirmation of successful transduction. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively.

In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The MISSION TRC3 ORF GFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Application

Functional Genomics/Overexpression Screening/Target Validation

Legal Information

Use of this product is subject to one or more license agreements. For details, please see the MISSION License Page
MISSION is a registered trademark of Sigma-Aldrich Co. LLC
TurboGFP is a trademark of Evrogen Co.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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