We are proud to offer the largest collection of fully sequenced and pre-cloned human open reading frames (ORFs) to-date, available in a pooled lentiviral whole genome format. Developed at the Broad Institute, the MISSION® TRC3 human whole genome pooled ORF library consists of over 17,000 unique ORF clones, encoding for over 14,000 genes used for overexpression screening. This exclusive library contains a unique 24 nucleotide barcode sequence associated with each ORF clone for easy hit identification and deep sequencing/deconvolution.
This ready-to-use MISSION TRC3 Human LentiORF pooled library allow for stable integration, enrichment of cells, and long-term gene expression in difficult-to-transfect cell lines utilizing our best-in-class lentiviral manufacturing. This collection provides researchers with the unique tools to gain insights into gene function through modulating gene and protein expression.
The classical genetic approach for exploring biological pathways typically begins by identifying mutations that cause a phenotype of interest. Overexpression or misexpression of a wild-type gene product, however, can also cause mutant phenotypes, providing geneticists with an alternative yet powerful tool to identify pathway components that might remain undetected using traditional loss-of-function analysis. The whole genome lentiviral ORF pool fills this gap and provides researchers with a powerful research tool for carrying out crucial biological experiments.
Whether looking to perform gene overexpression studies or validate gene knockdown/knockout results from an RNAi or CRISPR experiment, LentiORFs are the ideal shortcut to protein expression and tool for gene analysis. Researchers also need to validate “hits” from a screen to confirm to eliminate false positives. This means confirming whether the observed phenotype is due the target gene of interest. Re-expressing the gene of interest via lentiORFs is the most accepted way to validate a gene target.
MISSION TRC3 Human LentiORF pooled whole genome library is provided in 8×25 μl aliquots at a minimum titer of 5×108 TU/ml (measured by a p24 assay).
This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling. Follow all published RGL-2 guidelines for laboratory handling and waste decontamination.