The recommended storage temperature for this product is for –20°C. The stability of this product at –80°C has not been evaluated. This compound is not directly soluble in water. However, product R9532, Pyocyanin, Ready Made Solution, is a 5 mg/mL solution in DMSO. This item has a general shelf live of 2 years at –20°C. The prepared solution is not stable when stored at refrigerated temperatures. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/429/027/p0046dat.pdf
P0046
Pyocyanin
from Pseudomonas aeruginosa, ≥98% (HPLC), solid, redox-active phenazine
Synonym(s):
5-Methyl-1(5H)-phenazinone, Pyocyanine, Sanasin, Sanazin
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About This Item
Empirical Formula (Hill Notation):
C13H10N2O
CAS Number:
Molecular Weight:
210.23
MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:
NACRES:
NA.77
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Product Name
Pyocyanin, from Pseudomonas aeruginosa, ≥98% (HPLC)
biological source
Pseudomonas aeruginosa
Quality Level
assay
≥98% (HPLC)
form
solid
solubility
DMSO: soluble
acetone: soluble
chloroform: soluble
ethanol: soluble
shipped in
wet ice
storage temp.
−20°C
SMILES string
CN1c2ccccc2N=C3C(=O)C=CC=C13
InChI
1S/C13H10N2O/c1-15-10-6-3-2-5-9(10)14-13-11(15)7-4-8-12(13)16/h2-8H,1H3
InChI key
YNCMLFHHXWETLD-UHFFFAOYSA-N
Related Categories
1 of 4
This Item | R9532 | S5192 | UC175 |
|---|---|---|---|
| assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay ≥98% (HPLC) |
| form solid | form DMSO solution | form solid | form solid |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| solubility DMSO: soluble, chloroform: soluble, acetone: soluble, ethanol: soluble | solubility - | solubility DMSO: soluble ~28 mg/mL, H2O: insoluble | solubility DMSO: soluble |
| biological source Pseudomonas aeruginosa | biological source - | biological source - | biological source - |
Biochem/physiol Actions
Pyocyanin, a blue-green pigment belonging to phenazine pigments, is a redox-active phenazine. Pyocyanin is an electron receptor, which stimulates redox cycling in bacteria, liver cells, and human epithlial cell lines.
Pyocyanin, a blue-green pigment belonging to phenazine pigments, is a redox-active phenazine. Pyocyanin is an electron receptor, which stimulates redox cycling in bacteria, liver cells, and human epithlial cell lines. Pyocyanin enhances the oxidative metabolism, which in turn increases the formation of intracellular reactive oxygen species (ROS) via reduction of NADPH. Pyocyanin also increases the release of IL-8 by airway epithelial cells both in vitro and in vivo. This involves signal transduction pathways that include oxidants, protein tyrosine kinases, and MAP kinases. IL-8 secretion by these cells is in synergy with inflammatory cytokines. Pyocyanin has been shown to accelerate neutrophil apoptosis in vitro, resulting in resolution of acute inflammation, which is beneficial for bacteria survival.
Preparation Note
For the preparation of an aqueous solution, dissolve pyocyanin in ethanol using 10% of the final required volume and then add distilled water to complete the volume.
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Fast selective detection of pyocyanin using cyclic voltammetry
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Microbial biotechnology, 13(4), 1132-1144 (2020-04-05)
Pf prophages are ssDNA filamentous prophages that are prevalent among various Pseudomonas aeruginosa strains. The genomes of Pf prophages contain not only core genes encoding functions involved in phage replication, structure and assembly but also accessory genes. By studying the
Theerthankar Das et al.
Frontiers in microbiology, 8, 2429-2429 (2018-01-10)
Pyocyanin secreted by
Mariola Andrejko et al.
Acta biochimica Polonica, 66(1), 91-100 (2019-03-05)
Thermally induced unfolding and renaturation capability of alkaline proteases (AprA) of three Pseudomonas aeruginosa strains, i.e. ATCC 27853 and two clinical isolates, was examined. Sequence analyses demonstrated a high level of aprA genes identity (99.24-99.8%) in these bacterial strains. The
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①試薬の保存温度として―８０℃は不適切でしょうか。 ②純水に溶解後の保存方法として、冷蔵保存することは可能でしょうか。 宜しくお願いいたします。
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