Product P0065 (Phospholipase D) is sold by activity units per milliliter (units/mL). The protein amount is not measured; only the units/mL are provided for this product.
P0065
Phospholipase D from Streptomyces chromofuscus
≥50,000 units/mL, buffered aqueous glycerol solution
Synonym(s):
Lecithinase D, Phosphatidylcholine phosphatidohydrolase
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form
buffered aqueous glycerol solution
Quality Level
specific activity
≥50,000 units/mL
mol wt
~60 kDa
shipped in
dry ice
storage temp.
−70°C
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This Item | P4912 | 525200-M | P0515 |
|---|---|---|---|
| specific activity ≥50,000 units/mL | specific activity ≥150 units/mg solid | specific activity ≥50 units/mg dry wt. | specific activity ≥60 units/mg protein |
| form buffered aqueous glycerol solution | form lyophilized powder | form lyophilized solid | form lyophilized powder |
| shipped in dry ice | shipped in - | shipped in - | shipped in - |
| mol wt ~60 kDa | mol wt - | mol wt - | mol wt - |
| storage temp. −70°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
General description
Phospholipase D (phosphatidylcholine cholinehydrolase, EC 3.1.4.4) is a ubiquitous enzyme with a molecular weight of ~ 50,000Da. It is expressed in a variety of plant tissues, few microorganisms and some mammalian tissues.[1]
Application
Biochem/physiol Actions
Phospholipase D (PLD) catalyses the hydrolysis of phosphatidylcholines (PC) to yield phosphatidic acid (PA) and choline.[4] PC-PLD activity is regulated by hormones, cytokines, neurotransmitters and growth factors. Thus, it is considered to have a role in signal transduction in various cell types. Three vital lipid messengers produced by PLD plays a vital role in various crucial cellular processes and these lipids also have an essential role in downstream triglyceride and phospholipid synthetic pathway.[5] PLD is essential for exocytosis in neurons and endocrine cells. In addition, it also plays a key role in phagocytosis.[6]
Phospholipase D is active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Physical form
Solution in 100 mM Tris/HCl, pH 8.0, 10% glycerol (v/v), and 0.1% Triton X-100 (w/v).
Other Notes
One unit will release 1 μmol of choline from L-α-phosphatidylcholine per hour at pH 8.0 and 30 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)
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Phospholipase D: a downstream effector of ARF in granulocytes.
Cockcroft S
Science, 263(5146), 523-526 (1994)
Lecithin: a by-product of biodiesel production and a source of choline for dairy cows
Italian Journal of Animal Science, 11:2 (2012)
Dynamics and function of phospholipase D and phosphatidic acid during phagocytosis.
Traffic, 7(3), 365-377 (2006)
Eliminating office barriers to the handicapped patient.
Henson JL
The New dentist, 9(6), 30-32 (1979)
Phospholipase D: enzymology, mechanisms of regulation, and function.
Exton JH
Physiological Reviews, 77(2), 303-320 (1997)
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