- P0065
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P0065
Phospholipase D from Streptomyces chromofuscus
≥50,000 units/mL, buffered aqueous glycerol solution
All Photos(1)
Recommended Products
form
buffered aqueous glycerol solution
Quality Level
specific activity
≥50,000 units/mL
mol wt
~60 kDa
shipped in
dry ice
storage temp.
−70°C
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This Item | P0652 | SRE0047 | G3651 |
|---|---|---|---|
| form buffered aqueous glycerol solution | form powder | form lyophilized powder | form lyophilized powder |
| specific activity ≥50,000 units/mL | specific activity ≥15 units/mg solid | specific activity ≥30 units/mg protein | specific activity ≥50 units/mg protein |
| mol wt ~60 kDa | mol wt ~50 kDa | mol wt 28.93 kDa | mol wt - |
| shipped in dry ice | shipped in - | shipped in wet ice | shipped in - |
| storage temp. −70°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 400 | Quality Level 200 |
General description
Phospholipase D (phosphatidylcholine cholinehydrolase, EC 3.1.4.4) is a ubiquitous enzyme with a molecular weight of ~ 50,000Da. It is expressed in a variety of plant tissues, few microorganisms and some mammalian tissues.
Application
Phospholipase D from Streptomyces chromofuscus has been used to cleave choline-containing phospholipids (PCs).
Biochem/physiol Actions
Phospholipase D (PLD) catalyses the hydrolysis of phosphatidylcholines (PC) to yield phosphatidic acid (PA) and choline. PC-PLD activity is regulated by hormones, cytokines, neurotransmitters and growth factors. Thus, it is considered to have a role in signal transduction in various cell types. Three vital lipid messengers produced by PLD plays a vital role in various crucial cellular processes and these lipids also have an essential role in downstream triglyceride and phospholipid synthetic pathway. PLD is essential for exocytosis in neurons and endocrine cells. In addition, it also plays a key role in phagocytosis.
Phospholipase D is active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Unit Definition
One unit will release 1 μmol of choline from L-α-phosphatidylcholine per hour at pH 8.0 and 37 °C.
Physical form
Solution in 100 mM Tris/HCl, pH 8.0, 10% glycerol (v/v), and 0.1% Triton X-100 (w/v).
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificate of Analysis
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Certificate of Origin
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