Phospholipase D from Streptomyces chromofuscus

≥50,000 units/mL, buffered aqueous glycerol solution

Phosphatidylcholine phosphatidohydrolase, Lecithinase D
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:


buffered aqueous glycerol solution

Quality Level

specific activity

≥50,000 units/mL

mol wt

~60 kDa

shipped in

dry ice

storage temp.


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General description

Phospholipase D (phosphatidylcholine cholinehydrolase, EC is a ubiquitous enzyme with a molecular weight of ~ 50,000Da. It is expressed in a variety of plant tissues, few microorganisms and some mammalian tissues.


Phospholipase D from Streptomyces chromofuscus has been used to cleave choline-containing phospholipids (PCs).

Biochem/physiol Actions

Phospholipase D (PLD) catalyses the hydrolysis of phosphatidylcholines (PC) to yield phosphatidic acid (PA) and choline. PC-PLD activity is regulated by hormones, cytokines, neurotransmitters and growth factors. Thus, it is considered to have a role in signal transduction in various cell types. Three vital lipid messengers produced by PLD plays a vital role in various crucial cellular processes and these lipids also have an essential role in downstream triglyceride and phospholipid synthetic pathway. PLD is essential for exocytosis in neurons and endocrine cells. In addition, it also plays a key role in phagocytosis.
Phospholipase D is active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.

Unit Definition

One unit will release 1 μmol of choline from L-α-phosphatidylcholine per hour at pH 8.0 and 37 °C.

Physical form

Solution in 100 mM Tris/HCl, pH 8.0, 10% glycerol (v/v), and 0.1% Triton X-100 (w/v).


Health hazard

Signal Word


Hazard Statements

Precautionary Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves


NONH for all modes of transport

WGK Germany


Certificate of Analysis

Certificate of Origin

Phospholipase D: a downstream effector of ARF in granulocytes.
Cockcroft S
Science, 263(5146), 523-526 (1994)
Lecithin: a by-product of biodiesel production and a source of choline for dairy cows
Italian Journal of Animal Science, 11:2 (2012)
Eliminating office barriers to the handicapped patient.
Henson JL
The New dentist, 9(6), 30-32 (1979)
Dynamics and function of phospholipase D and phosphatidic acid during phagocytosis.
Traffic, 7(3), 365-377 (2006)
Purification of Streptomyces chromofuscus phospholipase D by hydrophobic affinity chromatography on palmitoyl cellulose.
Imamura S and Horiuti Y
Journal of Biochemistry, 85(1), 79-95 (1979)

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