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P0114

Phosphatase, Alkaline from bovine intestinal mucosa

BioUltra, ≥5,700 DEA units/mg protein

Synonym(s):

Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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About This Item

CAS Number:
9001-78-9
UNSPSC Code:
12352204
eCl@ss:
42010105
NACRES:
NA.54
EC Number:
3.1.3.1 (BRENDA, IUBMB)
MDL number:
MFCD00131849
Specific activity:
≥5,700 DEA units/mg protein
Concentration:
≥10 mg/mL

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product line

BioUltra

form

(40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)

specific activity

≥5,700 DEA units/mg protein

mol wt

dimer ~160 kDa

concentration

≥10 mg/mL

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

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1 of 4

This Item
P5521P6774P7640
Phosphatase, Alkaline from bovine intestinal mucosa BioUltra, ≥5,700 DEA units/mg protein

Sigma-Aldrich

P0114

Phosphatase, Alkaline from bovine intestinal mucosa

Phosphatase, Alkaline from bovine intestinal mucosa ≥2,000 DEA units/mg protein

Sigma-Aldrich

P5521

Phosphatase, Alkaline from bovine intestinal mucosa

Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous solution, ≥2,000 DEA units/mg protein

Sigma-Aldrich

P6774

Phosphatase, Alkaline from bovine intestinal mucosa

Phosphatase, Alkaline from bovine intestinal mucosa lyophilized powder, ≥10 DEA units/mg solid

Sigma-Aldrich

P7640

Phosphatase, Alkaline from bovine intestinal mucosa

specific activity

≥5,700 DEA units/mg protein

specific activity

≥2,000 DEA units/mg protein

specific activity

≥2,000 DEA units/mg protein

specific activity

≥10 DEA units/mg solid

concentration

≥10 mg/mL

concentration

≥1.0 mg/mL

concentration

5-20 mg/mL

concentration

-

form

(40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)

form

aqueous solution

form

buffered aqueous solution

form

lyophilized powder

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

wet ice

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

mol wt

dimer ~160 kDa

mol wt

dimer ~160 kDa

mol wt

dimer ~160 kDa

mol wt

dimer ~160 kDa

General description

Alkaline phosphatase from bovine intestinal mucosa is both a phosphomonoesterase and a pyrophosphatase. [1]
Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.
Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity.

Application

Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..
High specific activity grade recommended for antibody and protein conjugation.
Phosphatase alkaline from bovine intestinal mucosa has been used in a study to measure the hydrolysis kinetics of three polyfluorinated alkyl phosphate monoesters, differing in fluorinated chain length. [2] It has also been used in a study to show that the developmentally controlled expression of the isosomes C-1 and C-2 indicate functional changes in intestinal alkaline phosphatase with tissue maturation. [3]

Biochem/physiol Actions

Alkaline phosphatase was shown to have anti-inflammatory effects and can reduce the severity of septic shock by detoxifying lipid A. [4][5]
The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.

Physical form

Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6

Analysis Note

Package sizes are based on DEA units

Other Notes

One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units)

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000533097
0000530648
0000530647
0000533097
0000530648

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Fuhong Su et al.
Critical care medicine, 34(8), 2182-2187 (2006-06-16)
Alkaline phosphatase may decrease the harmful effects of lipopolysaccharide by detoxifying lipid A. The aim of this study was to investigate whether administration of alkaline phosphatase is beneficial in a clinically relevant septic shock model. Interventional laboratory study. University hospital
Suzanne Kats et al.
Recent patents on inflammation & allergy drug discovery, 3(3), 214-220 (2009-06-19)
Laboratory and clinical data have implicated endotoxin as an important factor in the inflammatory response to cardiopulmonary bypass. Alkaline phosphatase prevents endotoxin-induced systemic inflammation in animals and humans. We assessed the effects of the administration of bovine intestinal alkaline phosphatase
Senem Aykul et al.
International journal of molecular sciences, 22(16) (2021-08-28)
Adipose tissues (AT) expand in response to energy surplus through adipocyte hypertrophy and hyperplasia. The latter, also known as adipogenesis, is a process by which multipotent precursors differentiate to form mature adipocytes. This process is directed by developmental cues that
M Besman et al.
The Journal of biological chemistry, 260(20), 11190-11193 (1985-09-15)
Alkaline phosphatases from calf and bovine small intestines have been isolated in homogeneous form from both mucosa and luminal contents. The detergent-solubilized calf enzyme resolves into two peaks of activity, C-1 and C-2, on chromatofocusing. Only one of these activity
Dagmara Wojcik-Grzybek et al.
International journal of molecular sciences, 23(6) (2022-03-26)
Inflammatory bowel diseases (IBD) are commonly considered as Crohn's disease and ulcerative colitis, but the possibility that the alterations in gut microbiota and oxidative stress may affect the course of experimental colitis in obese physically exercising mice treated with the
View All Related Papers

Protocols

Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

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