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P0270

Sigma-Aldrich

Calmodulin from bovine heart

lyophilized powder, 2,500-10,000 units/mg protein

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Synonym(s):
Phosphodiesterase 3′:5′-cyclic nucleotide activator from bovine heart
CAS Number:
MDL number:
NACRES:
NA.61

biological source

bovine heart

Quality Level

form

lyophilized powder

specific activity

2,500-10,000 units/mg protein

composition

Protein, ≥85% Lowry

technique(s)

calcium flux assay: suitable

UniProt accession no.

storage temp.

−20°C

Gene Information

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This Item
C4874P1431P5511
Calmodulin bovine recombinant, expressed in E. coli, lyophilized powder, ≥98% (SDS-PAGE)

Sigma-Aldrich

C4874

Calmodulin bovine

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

specific activity

2,500-10,000 units/mg protein

specific activity

-

specific activity

-

specific activity

≥0.8 units/μg protein

technique(s)

calcium flux assay: suitable

technique(s)

-

technique(s)

ligand binding assay: suitable

technique(s)

-

UniProt accession no.

P62157

UniProt accession no.

P62157

UniProt accession no.

P62157

UniProt accession no.

P00517, P05131

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Application

This is the protein activator required for the Ca2+ dependent activation of phosphodiesterase, 3′:5′-cyclic nucleotide, P0520.

Biochem/physiol Actions

Ca2+ binding protein that is required for activation of cyclic nucleotide-dependent phosphodiesterase. It is also a cofactor/activator of nitric oxide synthase, calcineurin, and many kinases including ATPase, myosin light chain kinase, and CAM kinase I, II, and III. It mediates ryanodine receptor activation by cyclic ADP ribose and is involved in intracellular Ca2+ homeostasis.

Unit Definition

One unit will stimulate 0.008 activated unit of phosphodiesterase, 3′:5′-cyclic nucleotide, P0520, in a 3 mL reaction volume at pH 7.5 and 30 °C, to 50% of the maximum activity of the enzyme when saturated with activator, in the presence of 0.01 mM Ca2+.

Physical form

Lyophilized powder containing buffer salts as imidazole and magnesium sulfate

Preparation Note

Purified by a modification of the procedure of Teo, T.S., et al., J. Biol. Chem., 248, 588 (1973).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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